| Title |
Comparison of analytical methods for profiling N- and O-linked glycans from cultured cell lines
|
|---|---|
| Published in |
Glycoconjugate Journal, October 2015
|
| DOI | 10.1007/s10719-015-9625-3 |
| Pubmed ID | |
| Authors |
Hiromi Ito, Hiroyuki Kaji, Akira Togayachi, Parastoo Azadi, Mayumi Ishihara, Rudolf Geyer, Christina Galuska, Hildegard Geyer, Kazuaki Kakehi, Mitsuhiro Kinoshita, Niclas G. Karlsson, Chunsheng Jin, Koichi Kato, Hirokazu Yagi, Sachiko Kondo, Nana Kawasaki, Noritaka Hashii, Daniel Kolarich, Kathrin Stavenhagen, Nicolle H. Packer, Morten Thaysen-Andersen, Miyako Nakano, Naoyuki Taniguchi, Ayako Kurimoto, Yoshinao Wada, Michiko Tajiri, Pengyuan Yang, Weiqian Cao, Hong Li, Pauline M. Rudd, Hisashi Narimatsu |
| Abstract |
The Human Disease Glycomics/Proteome Initiative (HGPI) is an activity in the Human Proteome Organization (HUPO) supported by leading researchers from international institutes and aims at development of disease-related glycomics/glycoproteomics analysis techniques. Since 2004, the initiative has conducted three pilot studies. The first two were N- and O-glycan analyses of purified transferrin and immunoglobulin-G and assessed the most appropriate analytical approach employed at the time. This paper describes the third study, which was conducted to compare different approaches for quantitation of N- and O-linked glycans attached to proteins in crude biological samples. The preliminary analysis on cell pellets resulted in wildly varied glycan profiles, which was probably the consequence of variations in the pre-processing sample preparation methodologies. However, the reproducibility of the data was not improved dramatically in the subsequent analysis on cell lysate fractions prepared in a specified method by one lab. The study demonstrated the difficulty of carrying out a complete analysis of the glycome in crude samples by any single technology and the importance of rigorous optimization of the course of analysis from preprocessing to data interpretation. It suggests that another collaborative study employing the latest technologies in this rapidly evolving field will help to realize the requirements of carrying out the large-scale analysis of glycoproteins in complex cell samples. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Japan | 1 | 33% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Croatia | 1 | 2% |
| Netherlands | 1 | 2% |
| Qatar | 1 | 2% |
| Unknown | 57 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 16 | 27% |
| Researcher | 12 | 20% |
| Professor | 6 | 10% |
| Student > Master | 5 | 8% |
| Other | 4 | 7% |
| Other | 12 | 20% |
| Unknown | 5 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 18 | 30% |
| Agricultural and Biological Sciences | 10 | 17% |
| Chemistry | 7 | 12% |
| Medicine and Dentistry | 5 | 8% |
| Computer Science | 2 | 3% |
| Other | 10 | 17% |
| Unknown | 8 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 May 2018.
All research outputs
#15,739,010
of 25,371,288 outputs
Outputs from Glycoconjugate Journal
#648
of 929 outputs
Outputs of similar age
#152,318
of 295,274 outputs
Outputs of similar age from Glycoconjugate Journal
#8
of 12 outputs
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