| Title |
SYBR Gold Fluorescence Quenching is a Sensitive Probe of Chitosan-microRNA Interactions
|
|---|---|
| Published in |
Journal of Fluorescence, October 2015
|
| DOI | 10.1007/s10895-015-1697-8 |
| Pubmed ID | |
| Authors |
Beatriz Santos-Carballal, Musti J. Swamy, Bruno M. Moerschbacher, Francisco M. Goycoolea |
| Abstract |
Competitive dye displacement titration has previously been used to characterize chitosan-DNA interactions using ethidium bromide. In this work, we aim to develop a fast and reliable method using SYBR Gold as a fluorescent probe to evaluate the binding affinity between ssRNA and chitosan. The interaction of chitosan with ssRNA was investigated as a function of temperature, molecular weight and degree of acetylation of chitosan, using competitive dye displacement titrations with fluorescence quenching. Affinity constants are reported, showing the high sensitivity of the interaction to the degree of acetylation of chitosan and barely dependent on the molecular weight. We propose that the mechanism of SYBR Gold fluorescence quenching is governed by both static and dynamic quenching. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 27% |
| Student > Doctoral Student | 4 | 18% |
| Researcher | 3 | 14% |
| Student > Master | 3 | 14% |
| Professor | 2 | 9% |
| Other | 1 | 5% |
| Unknown | 3 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 23% |
| Agricultural and Biological Sciences | 4 | 18% |
| Chemistry | 4 | 18% |
| Environmental Science | 1 | 5% |
| Immunology and Microbiology | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 4 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 October 2015.
All research outputs
#20,295,099
of 22,831,537 outputs
Outputs from Journal of Fluorescence
#354
of 425 outputs
Outputs of similar age
#238,738
of 284,638 outputs
Outputs of similar age from Journal of Fluorescence
#6
of 10 outputs
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