| Title |
A novel isothermal microcalorimetry tool to assess drug effects on Ancylostoma ceylanicum and Necator americanus
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, October 2015
|
| DOI | 10.1007/s00253-015-7081-4 |
| Pubmed ID | |
| Authors |
Dayana Flores, Gordana Panic, Olivier Braissant, Jennifer Keiser |
| Abstract |
Soil-transmitted helminths, which affect the poorest communities, worldwide cause a range of symptoms and morbidity, yet few treatment options are available and drug resistance is a concern. To improve and accelerate anthelminthic drug discovery, novel drug screening tools such as isothermal microcalorimetry (IMC) have been tested with great potential. In this study, we used a novel microcalorimeter, the calScreener™, to study the viability on the hookworms Necator americanus and Ancylostoma ceylanicum as well as the whipworm Trichuris muris. Significant heat flow signals could be obtained with already one adult worm per channel for all three species. High-amplitude oscillations were observed for the hookworms; however, adult T. muris showed a twofold heat flow decrease during the first 24 h. Antinematodal effects of ivermectin and levamisole at 1, 10, and 100 μg/ml were evaluated on adult N. americanus and A. ceylanicum. Levamisole-treated hookworms showed a decline in heat flow and oscillation amplitude in a dose-response manner. Heat flow for ivermectin-treated hookworms increased proportionally with increased concentrations of ivermectin, though the wavelet analysis showed an opposite trend as observed by flatter wavelets. In conclusion, the calScreener™ is an excellent tool to study drug effects on intestinal hookworms at the adult worm stage as it offers a lower detection limit than other IMC devices and the possibility to monitor worm viability online. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 46 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 2% |
| Unknown | 45 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 17% |
| Student > Master | 6 | 13% |
| Student > Bachelor | 6 | 13% |
| Researcher | 5 | 11% |
| Student > Doctoral Student | 2 | 4% |
| Other | 8 | 17% |
| Unknown | 11 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 26% |
| Medicine and Dentistry | 7 | 15% |
| Veterinary Science and Veterinary Medicine | 3 | 7% |
| Biochemistry, Genetics and Molecular Biology | 3 | 7% |
| Psychology | 2 | 4% |
| Other | 7 | 15% |
| Unknown | 12 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 November 2015.
All research outputs
#18,922,529
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#6,246
of 8,034 outputs
Outputs of similar age
#196,910
of 289,369 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#81
of 140 outputs
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