| Title |
OsNDUFA9 encoding a mitochondrial complex I subunit is essential for embryo development and starch synthesis in rice
|
|---|---|
| Published in |
Plant Cell Reports, August 2018
|
| DOI | 10.1007/s00299-018-2338-x |
| Pubmed ID | |
| Authors |
Tingting Hu, Yunlu Tian, Jianping Zhu, Yunlong Wang, Ruonan Jing, Jie Lei, Yinglun Sun, Yanfang Yu, Jingfang Li, Xiaoli Chen, Xiaopin Zhu, Yuanyuan Hao, Linglong Liu, Yihua Wang, Jianmin Wan |
| Abstract |
Loss of function of a mitochondrial complex I subunit (OsNDUFA9) causes abnormal embryo development and affects starch synthesis by altering the expression of starch synthesis-related genes and proteins. Proton-pumping NADH: ubiquinone oxidoreductase (also called complex I) is thought to be the largest and most complicated enzyme of the mitochondrial respiratory chain. Mutations of complex I subunits have been revealed to link with a number of growth inhibitions in plants. However, the function of complex I subunits in rice remains unclear. Here, we isolated a rice floury endosperm mutant (named flo13) that was embryonic lethal and failed to germinate. Semi-thin sectioning analysis showed that compound starch grain development in the mutant was greatly impaired, leading to significantly compromised starch biosynthesis and decreased 1000-grain weight relative to the wild type. Map-based cloning revealed that FLO13 encodes an accessory subunit of complex I protein (designated as OsNDUFA9). A single nucleotide substitution (G18A) occurred in the first exon of OsNDUFA9, introducing a premature stop codon in the flo13 mutant gene. OsNDUFA9 was ubiquitously expressed in various tissues and the OsNDUFA9 protein was localized to the mitochondria. Quantitative RT-PCR and protein blotting indicated loss of function of OsNDUFA9 altered gene expression and protein accumulation associated with respiratory electron chain complex in the mitochondria. Moreover, transmission electron microscopic analysis showed that the mutant lacked obvious mitochondrial cristae structure in the mitochondria of endosperm cell. Our results demonstrate that the OsNDUFA9 subunit of complex I is essential for embryo development and starch synthesis in rice endosperm. |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 2 | 18% |
| Mexico | 1 | 9% |
| Ireland | 1 | 9% |
| United States | 1 | 9% |
| Canada | 1 | 9% |
| Unknown | 5 | 45% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 55% |
| Scientists | 5 | 45% |
Mendeley readers
The data shown below were compiled from readership statistics for 18 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 18 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 11% |
| Student > Master | 2 | 11% |
| Lecturer | 1 | 6% |
| Student > Bachelor | 1 | 6% |
| Other | 1 | 6% |
| Other | 3 | 17% |
| Unknown | 8 | 44% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 33% |
| Biochemistry, Genetics and Molecular Biology | 2 | 11% |
| Philosophy | 1 | 6% |
| Mathematics | 1 | 6% |
| Unknown | 8 | 44% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 December 2021.
All research outputs
#6,551,309
of 23,914,147 outputs
Outputs from Plant Cell Reports
#631
of 2,266 outputs
Outputs of similar age
#110,872
of 337,977 outputs
Outputs of similar age from Plant Cell Reports
#15
of 28 outputs
Altmetric has tracked 23,914,147 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 2,266 research outputs from this source. They receive a mean Attention Score of 4.1. This one has gotten more attention than average, scoring higher than 71% of its peers.
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We're also able to compare this research output to 28 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.