Title |
Distinct routes of lineage development reshape the human blood hierarchy across ontogeny
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Published in |
Science, November 2015
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DOI | 10.1126/science.aab2116 |
Pubmed ID | |
Authors |
Faiyaz Notta, Sasan Zandi, Naoya Takayama, Stephanie Dobson, Olga I Gan, Gavin Wilson, Kerstin B Kaufmann, Jessica McLeod, Elisa Laurenti, Cyrille F Dunant, John D McPherson, Lincoln D Stein, Yigal Dror, John E Dick |
Abstract |
In a classical view of hematopoiesis, the various blood cell lineages arise via a hierarchical scheme starting with multipotent stem cells that become increasingly restricted in their differentiation potential through oligopotent and then unipotent progenitors. We developed a cell-sorting scheme to resolve myeloid (My), erythroid (Er), and megakaryocytic (Mk) fates from single CD34+ cells and then mapped the progenitor hierarchy across human development. Fetal liver contained large numbers of distinct oligopotent progenitors with intermingled My, Er, and Mk fates. However, few oligopotent progenitor intermediates were present in the adult bone marrow. Instead only two progenitor classes predominate, multipotent and unipotent, with Er-Mk lineages emerging from multipotent cells. The developmental shift to an adult "two-tier" hierarchy challenges current dogma and provides a revised framework to understand normal and disease states of human hematopoiesis. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 16 | 25% |
Canada | 8 | 13% |
United Kingdom | 6 | 9% |
Australia | 4 | 6% |
Spain | 3 | 5% |
Belgium | 1 | 2% |
Russia | 1 | 2% |
Chile | 1 | 2% |
Portugal | 1 | 2% |
Other | 5 | 8% |
Unknown | 18 | 28% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 32 | 50% |
Scientists | 23 | 36% |
Science communicators (journalists, bloggers, editors) | 6 | 9% |
Practitioners (doctors, other healthcare professionals) | 3 | 5% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 6 | <1% |
United Kingdom | 5 | <1% |
Japan | 3 | <1% |
Germany | 2 | <1% |
Canada | 2 | <1% |
France | 2 | <1% |
Italy | 1 | <1% |
Sweden | 1 | <1% |
Finland | 1 | <1% |
Other | 1 | <1% |
Unknown | 1118 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 288 | 25% |
Researcher | 213 | 19% |
Student > Master | 129 | 11% |
Student > Bachelor | 97 | 8% |
Student > Doctoral Student | 71 | 6% |
Other | 165 | 14% |
Unknown | 179 | 16% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 249 | 22% |
Medicine and Dentistry | 139 | 12% |
Immunology and Microbiology | 97 | 8% |
Engineering | 13 | 1% |
Other | 67 | 6% |
Unknown | 202 | 18% |