Title |
Strain Improvement of Streptomyces xanthochromogenes RIA 1098 for Enhanced Pravastatin Production at High Compactin Concentrations
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Published in |
Indian Journal of Microbiology, May 2015
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DOI | 10.1007/s12088-015-0537-5 |
Pubmed ID | |
Authors |
Vakhtang V. Dzhavakhiya, Tatiana M. Voinova, Elena V. Glagoleva, Dmitry V. Petukhov, Alexander I. Ovchinnikov, Maksim I. Kartashov, Boris B. Kuznetsov, Konstantin G. Skryabin |
Abstract |
Pravastatin is one of the most popular cholesterol-lowering drugs. Its industrial production represents a two-stage process including the microbial production of compactin and its further biocatalytic conversion to pravastatin. To increase a conversion rate, a higher compactin content in fermentation medium should be used; however, high compactin concentrations inhibit microbial growth. Therefore, the improvement of the compactin resistance of a producer still remains a relevant problem. A multi-step random UV mutagenesis of a Streptomyces xanthochromogenes strain RIA 1098 and the further selection of high-yield compactin-resistant mutants have resulted in a highly productive compactin-resistant strain S 33-1. After the fermentation medium improvement, the maximum bioconversion rate of this strain has reached 91 % at the daily compactin dose equal to 1 g/L and still remained high (83 %) even at the doubled dose (2 g/L). A 1-year study of the mutant strain stability has proved a stable inheritance of its characteristics that provides this strain to be very promising for the pravastatin-producing industry. |
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