| Title |
Protein kinase R (PKR) plays a pro-viral role in porcine reproductive and respiratory syndrome virus (PRRSV) replication by modulating viral gene transcription
|
|---|---|
| Published in |
Archives of Virology, November 2015
|
| DOI | 10.1007/s00705-015-2671-0 |
| Pubmed ID | |
| Authors |
Xiuqing Wang, Hanmo Zhang, Alex M. Abel, Eric Nelson |
| Abstract |
Protein kinase R (PKR) is involved in apoptotic cell death and antiviral activities in response to many virus infections. To reveal the role of PKR in the replication of porcine reproductive and respiratory syndrome virus (PRRSV), we first examined the kinetics of PKR phosphorylation during PRRSV infection. The results showed that PRRSV transiently activates PKR at 12 and 24 h postinfection. Surprisingly, eIF-2α, the well-known downstream target of PKR, was significantly phosphorylated compared to mock-infected cells only at 48 and 72 h postinfection. Reduced viral gene transcription, viral protein synthesis, and virus titer were detected in cells transfected with PKR silencing RNA prior to PRRSV infection compared to control silencing RNA transfected cells, indicating a role of PKR in facilitating virus replication. Overall, our data suggest that PKR is not a major contributor to the phosphorylation of eIF-2α during PRRSV infection, but it plays a pro-viral role in PRRSV replication by modulating primarily viral gene transcription. |
Mendeley readers
The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 4 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 50% |
| Researcher | 1 | 25% |
| Lecturer > Senior Lecturer | 1 | 25% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 50% |
| Veterinary Science and Veterinary Medicine | 1 | 25% |
| Unknown | 1 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2017.
All research outputs
#7,468,612
of 22,832,057 outputs
Outputs from Archives of Virology
#918
of 4,159 outputs
Outputs of similar age
#96,811
of 285,879 outputs
Outputs of similar age from Archives of Virology
#13
of 74 outputs
Altmetric has tracked 22,832,057 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
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We're also able to compare this research output to 74 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 79% of its contemporaries.