| Title |
A Trimodal Imaging Platform for Tracking Viable Transplanted Pancreatic Islets In Vivo: F-19 MR, Fluorescence, and Bioluminescence Imaging
|
|---|---|
| Published in |
Molecular Imaging and Biology, August 2018
|
| DOI | 10.1007/s11307-018-1270-3 |
| Pubmed ID | |
| Authors |
A. Gálisová, V. Herynek, E. Swider, E. Sticová, A. Pátiková, L. Kosinová, J. Kříž, M. Hájek, M. Srinivas, D. Jirák |
| Abstract |
Combining specific and quantitative F-19 magnetic resonance imaging (MRI) with sensitive and convenient optical imaging provides complementary information about the distribution and viability of transplanted pancreatic islet grafts. In this study, pancreatic islets (PIs) were labeled with positively charged multimodal nanoparticles based on poly(lactic-co-glycolic acid) (PLGA-NPs) with encapsulated perfluoro-15-crown-5-ether and the near-infrared fluorescent dye indocyanine green. One thousand and three thousand bioluminescent PIs were transplanted into subcutaneous artificial scaffolds, which served as an alternative transplant site. The grafts were monitored using in vivo F-19 MR, fluorescence, and bioluminescence imaging in healthy rats for 2 weeks. Transplanted PIs were unambiguously localized in the scaffolds by F-19 MRI throughout the whole experiment. Fluorescence was detected in the first 4 days after transplantation only. Importantly, in vivo bioluminescence correlated with the F-19 MRI signal. We developed a trimodal imaging platform for in vivo examination of transplanted PIs. Fluorescence imaging revealed instability of the fluorescent dye and its limited applicability for longitudinal in vivo studies. A correlation between the bioluminescence signal and the F-19 MRI signal indicated the fast clearance of PLGA-NPs from the transplantation site after cell death, which addresses a major issue with intracellular imaging labels. Therefore, the proposed PLGA-NP platform is reliable for reflecting the status of transplanted PIs in vivo. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 50% |
| United States | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 37 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 22% |
| Researcher | 6 | 16% |
| Student > Doctoral Student | 3 | 8% |
| Student > Master | 3 | 8% |
| Student > Bachelor | 2 | 5% |
| Other | 2 | 5% |
| Unknown | 13 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 8 | 22% |
| Biochemistry, Genetics and Molecular Biology | 5 | 14% |
| Medicine and Dentistry | 5 | 14% |
| Agricultural and Biological Sciences | 2 | 5% |
| Veterinary Science and Veterinary Medicine | 1 | 3% |
| Other | 2 | 5% |
| Unknown | 14 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 16. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 June 2019.
All research outputs
#2,261,846
of 25,385,509 outputs
Outputs from Molecular Imaging and Biology
#21
of 837 outputs
Outputs of similar age
#45,440
of 344,376 outputs
Outputs of similar age from Molecular Imaging and Biology
#2
of 16 outputs
Altmetric has tracked 25,385,509 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 837 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.0. This one has done particularly well, scoring higher than 97% of its peers.
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We're also able to compare this research output to 16 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 87% of its contemporaries.