| Title |
Purification of virus-like particles (VLPs) expressed in the silkworm Bombyx mori
|
|---|---|
| Published in |
Biotechnology Techniques, January 2018
|
| DOI | 10.1007/s10529-018-2516-5 |
| Pubmed ID | |
| Authors |
Robert Minkner, Enoch Y. Park |
| Abstract |
Virus-like particles (VLPs) are a promising and developing option for vaccination and gene therapy. They are also interesting as shuttles for drug targeting. Currently, several different gene expression systems are available, among which the silkworm expression system is known for its mass production capacity. However, cost-effective purification with high purity of the target protein is a particular bottleneck for this system. The present review evaluates the advances in the purification of VLPs, especially from silkworm larval hemolymph. Beginning with applicable pre-treatments for VLPs over to chromatography methods and quality control of the purified VLPs. Whereupon the main focus is on the different chromatography approaches for the purification, but the structure of the VLPs and their intended use for humans make also the quality control important. Within this, the stability of the VLPs which has to be considered for the purification is as well discussed. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 21% |
| Student > Ph. D. Student | 4 | 14% |
| Student > Master | 2 | 7% |
| Professor | 1 | 4% |
| Unspecified | 1 | 4% |
| Other | 2 | 7% |
| Unknown | 12 | 43% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 5 | 18% |
| Biochemistry, Genetics and Molecular Biology | 3 | 11% |
| Chemical Engineering | 2 | 7% |
| Unspecified | 1 | 4% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
| Other | 2 | 7% |
| Unknown | 14 | 50% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 September 2018.
All research outputs
#22,767,715
of 25,382,440 outputs
Outputs from Biotechnology Techniques
#2,493
of 2,762 outputs
Outputs of similar age
#389,793
of 449,219 outputs
Outputs of similar age from Biotechnology Techniques
#14
of 17 outputs
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