| Title |
A Novel Molecular Design for a Hybrid Phage-DNA Construct Against DKK1
|
|---|---|
| Published in |
Molecular Biotechnology, September 2018
|
| DOI | 10.1007/s12033-018-0115-2 |
| Pubmed ID | |
| Authors |
Saeed Khalili, Mohamad Javad Rasaee, Taravat Bamdad, Maysam Mard-Soltani, Majid Asadi Ghalehni, Abolfazl Jahangiri, Mohammad Hassan Pouriayevali, Mohammad Reza Aghasadeghi, Fatemeh Malaei |
| Abstract |
Nucleic acid immunization has recently exhibited a great promise for immunotherapy of various diseases. However, it is now clear that powerful strategies are imminently needed to improve their efficiency. In this regard, whole bacteriophage particles have been described as efficient DNA vaccine delivery vehicles, capable of circumventing the limitations of naked DNA immunization. Moreover, phage particles could be engineered to display specific peptides on their surfaces. Given these inherent characteristics of phages, we have designed a novel hybrid phage-DNA immunization vector using both M13 and pAAV plasmid elements. Following the construction and in vitro confirmation of the designed vectors, they were used for comparative mice immunization, carrying the same DNA sequence. The results indicated the efficacy of the designed hybrid phage particles, to elicit higher humoral immunity, in comparison to conventional DNA-immunization vectors (pCI). In light of these findings, it could be concluded that using adeno-associated virus (AAV) expression cassette along with displaying TAT peptide on the surface of the phage particle could be deemed as an appealing strategy to enhance the DNA-immunization and vaccination efficacy. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 19 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 32% |
| Student > Master | 3 | 16% |
| Researcher | 2 | 11% |
| Student > Ph. D. Student | 1 | 5% |
| Other | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 6 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 5 | 26% |
| Biochemistry, Genetics and Molecular Biology | 4 | 21% |
| Veterinary Science and Veterinary Medicine | 1 | 5% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
| Agricultural and Biological Sciences | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 6 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 September 2018.
All research outputs
#20,532,290
of 23,102,082 outputs
Outputs from Molecular Biotechnology
#820
of 981 outputs
Outputs of similar age
#292,036
of 335,392 outputs
Outputs of similar age from Molecular Biotechnology
#9
of 17 outputs
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