| Title |
Regulation signature of miR-143 and miR-26 in porcine Salmonella infection identified by binding site enrichment analysis
|
|---|---|
| Published in |
Molecular Genetics and Genomics, November 2015
|
| DOI | 10.1007/s00438-015-1146-z |
| Pubmed ID | |
| Authors |
Min Yao, Weihua Gao, Hengxun Tao, Jun Yang, Guoping Liu, Tinghua Huang |
| Abstract |
Salmonella infects many vertebrate species, and pigs colonized with Salmonella are typically Salmonella carriers. Transcriptomic analysis of the response to Salmonella infection in whole blood has been reported for the pig. The objective of this study is to identify the important miRNAs involved in Salmonella infection using binding site enrichment analysis. We predicted porcine microRNA (miRNA) binding sites in the 3' UTR of protein-coding genes for all miRNA families. Based on those predictions, we analyzed miRNA-binding sites for mRNAs expressed in peripheral blood to investigate the functional importance of miRNAs in Salmonella infection in pig. Enrichment analysis revealed that binding sites of five miRNAs (including miR-143, -9839, -26, -2483, and -4335) were significantly over represented for the differentially expressed gene sets. Real-time PCR results indicated that selected members of this miRNA group (miR-143, -26, and -4335) were differentially expressed in whole blood after Salmonella inoculation. The luciferase reporter assay showed that ATP6V1A and IL13RA1 were targets of miR-143 and that miR-26 regulates BINP3L and ARL6IP6. The results strongly suggest that miR-143 and miR-26 play important regulatory roles in the development of Salmonella infection in pig. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 33% |
| Unknown | 2 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 33% |
| Members of the public | 1 | 33% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 10 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 4 | 40% |
| Student > Master | 2 | 20% |
| Student > Bachelor | 2 | 20% |
| Student > Ph. D. Student | 1 | 10% |
| Unspecified | 1 | 10% |
| Other | 0 | 0% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 40% |
| Agricultural and Biological Sciences | 2 | 20% |
| Nursing and Health Professions | 1 | 10% |
| Unspecified | 1 | 10% |
| Medicine and Dentistry | 1 | 10% |
| Other | 1 | 10% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 December 2015.
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#16,721,717
of 25,373,627 outputs
Outputs from Molecular Genetics and Genomics
#2,699
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#226,346
of 392,711 outputs
Outputs of similar age from Molecular Genetics and Genomics
#12
of 33 outputs
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