| Title |
Phenotypic and Physiological Characterization of the Epibiotic Interaction Between TM7x and Its Basibiont Actinomyces
|
|---|---|
| Published in |
Microbial Ecology, November 2015
|
| DOI | 10.1007/s00248-015-0711-7 |
| Pubmed ID | |
| Authors |
Batbileg Bor, Nicole Poweleit, Justin S. Bois, Lujia Cen, Joseph K. Bedree, Z. Hong Zhou, Robert P. Gunsalus, Renate Lux, Jeffrey S. McLean, Xuesong He, Wenyuan Shi |
| Abstract |
Despite many examples of obligate epibiotic symbiosis (one organism living on the surface of another) in nature, such an interaction has rarely been observed between two bacteria. Here, we further characterize a newly reported interaction between a human oral obligate parasitic bacterium TM7x (cultivated member of Candidatus Saccharimonas formerly Candidate Phylum TM7), and its basibiont Actinomyces odontolyticus species (XH001), providing a model system to study epiparasitic symbiosis in the domain Bacteria. Detailed microscopic studies indicate that both partners display extensive morphological changes during symbiotic growth. XH001 cells manifested as short rods in monoculture, but displayed elongated and hyphal morphology when physically associated with TM7x. Interestingly, these dramatic morphological changes in XH001 were also induced in oxygen-depleted conditions, even in the absence of TM7x. Targeted quantitative real-time PCR (qRT-PCR) analyses revealed that both the physical association with TM7x as well as oxygen depletion triggered up-regulation of key stress response genes in XH001, and in combination, these conditions act in an additive manner. TM7x and XH001 co-exist with relatively uniform cell morphologies under nutrient-replete conditions. However, upon nutrient depletion, TM7x-associated XH001 displayed a variety of cell morphologies, including swollen cell body, clubbed-ends, and even cell lysis, and a large portion of TM7x cells transformed from ultrasmall cocci into elongated cells. Our study demonstrates a highly dynamic interaction between epibiont TM7x and its basibiont XH001 in response to physical association or environmental cues such as oxygen level and nutritional status, as reflected by their morphological and physiological changes during symbiotic growth. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 3 | 50% |
| France | 1 | 17% |
| Ireland | 1 | 17% |
| Unknown | 1 | 17% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 4 | 67% |
| Science communicators (journalists, bloggers, editors) | 1 | 17% |
| Members of the public | 1 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 5% |
| France | 1 | 2% |
| Unknown | 41 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 30% |
| Researcher | 5 | 11% |
| Student > Bachelor | 3 | 7% |
| Professor | 3 | 7% |
| Professor > Associate Professor | 2 | 5% |
| Other | 4 | 9% |
| Unknown | 14 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 16 | 36% |
| Biochemistry, Genetics and Molecular Biology | 5 | 11% |
| Immunology and Microbiology | 4 | 9% |
| Medicine and Dentistry | 3 | 7% |
| Computer Science | 1 | 2% |
| Other | 1 | 2% |
| Unknown | 14 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 09 October 2019.
All research outputs
#4,669,325
of 22,833,393 outputs
Outputs from Microbial Ecology
#509
of 2,058 outputs
Outputs of similar age
#78,512
of 386,225 outputs
Outputs of similar age from Microbial Ecology
#7
of 34 outputs
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