| Title |
Construction and Characterization of Virus-Like Particles: A Review
|
|---|---|
| Published in |
Molecular Biotechnology, September 2012
|
| DOI | 10.1007/s12033-012-9598-4 |
| Pubmed ID | |
| Authors |
Andris Zeltins |
| Abstract |
Over the last three decades, virus-like particles (VLPs) have evolved to become a widely accepted technology, especially in the field of vaccinology. In fact, some VLP-based vaccines are currently used as commercial medical products, and other VLP-based products are at different stages of clinical study. Several remarkable advantages have been achieved in the development of VLPs as gene therapy tools and new nanomaterials. The analysis of published data reveals that at least 110 VLPs have been constructed from viruses belonging to 35 different families. This review therefore discusses the main principles in the cloning of viral structural genes, the relevant host systems and the purification procedures that have been developed. In addition, the methods that are used to characterize the structural integrity, stability, and components, including the encapsidated nucleic acids, of newly synthesized VLPs are analyzed. Moreover, some of the modifications that are required to construct VLP-based carriers of viral origin with defined properties are discussed, and examples are provided. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 820 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 3 | <1% |
| United States | 3 | <1% |
| Germany | 2 | <1% |
| India | 2 | <1% |
| Indonesia | 1 | <1% |
| France | 1 | <1% |
| Netherlands | 1 | <1% |
| Lithuania | 1 | <1% |
| Brazil | 1 | <1% |
| Other | 5 | <1% |
| Unknown | 800 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 145 | 18% |
| Student > Master | 141 | 17% |
| Student > Bachelor | 126 | 15% |
| Researcher | 98 | 12% |
| Student > Doctoral Student | 40 | 5% |
| Other | 77 | 9% |
| Unknown | 193 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 184 | 22% |
| Biochemistry, Genetics and Molecular Biology | 168 | 20% |
| Chemistry | 58 | 7% |
| Immunology and Microbiology | 38 | 5% |
| Engineering | 36 | 4% |
| Other | 122 | 15% |
| Unknown | 214 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 November 2023.
All research outputs
#1,989,795
of 24,250,928 outputs
Outputs from Molecular Biotechnology
#13
of 1,038 outputs
Outputs of similar age
#12,837
of 174,344 outputs
Outputs of similar age from Molecular Biotechnology
#2
of 9 outputs
Altmetric has tracked 24,250,928 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,038 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done particularly well, scoring higher than 98% of its peers.
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We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 7 of them.