| Title |
Comparative transcriptome analysis shows the defense response networks regulated by miR482b
|
|---|---|
| Published in |
Plant Cell Reports, September 2018
|
| DOI | 10.1007/s00299-018-2344-z |
| Pubmed ID | |
| Authors |
Ning Jiang, Jun Cui, Guanglei Yang, Xiaoli He, Jun Meng, Yushi Luan |
| Abstract |
The transcriptomic profile in the leaves of miR482b-overexpressing tomato plants revealed that miR482b may suppress alpha-linolenic acid metabolism, cysteine and methionine metabolism, plant-pathogen interaction, and the MAPK pathway to reduce resistance to Phytophthora infestans. Our previous study showed that tomato miR482b acted as a negative regulator during tomato resistance to Phytophthora infestans by silencing NBS-LRR genes. To investigate pathways related to miR482b, the transcriptomic profile of tomato plants that overexpressed miR482b was constructed. A total of 47,124,670 raw sequence reads from the leaves of miR482b-overexpressing tomato plants were generated by Illumina sequencing. A total of 746 genes in miR482b-overexpressing tomato plants were found to show significantly differential expression relative to those in wild-type tomato plants, including 132 up-regulated genes and 614 down-regulated genes. GO and KEGG enrichment analyses showed that plant-pathogen interaction, the MAPK pathway, and the pathways related to JA and ET biosynthesis were affected by miR482b in tomato. qRT-PCR results showed that all the enriched genes in these pathways were down-regulated in tomato plants that overexpressed miR482b and up-regulated in tomato plants that overexpressed an NBS-LRR gene (Soly02g036270.2, the target gene of miR482b). After P. infestans infection, the expression of the enriched genes showed a time-dependent response, and the genes played different roles between resistant tomato (Solanum pimpinellifolium L3708) and tomato susceptible to P. infestans (S. lycopersicum Zaofen No. 2). Our results have, therefore, demonstrated that miR482b is an important component of defense response network. This will also help to identify candidate genes involved in plant-pathogen interaction. |
X Demographics
The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 3 | 27% |
| United States | 2 | 18% |
| Canada | 1 | 9% |
| Saudi Arabia | 1 | 9% |
| Unknown | 4 | 36% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 6 | 55% |
| Members of the public | 5 | 45% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 23% |
| Researcher | 6 | 23% |
| Student > Doctoral Student | 3 | 12% |
| Student > Ph. D. Student | 3 | 12% |
| Professor | 2 | 8% |
| Other | 2 | 8% |
| Unknown | 4 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 12 | 46% |
| Biochemistry, Genetics and Molecular Biology | 7 | 27% |
| Computer Science | 2 | 8% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Chemistry | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 3 | 12% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 August 2022.
All research outputs
#5,116,983
of 24,363,506 outputs
Outputs from Plant Cell Reports
#332
of 2,296 outputs
Outputs of similar age
#94,474
of 339,776 outputs
Outputs of similar age from Plant Cell Reports
#10
of 27 outputs
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So far Altmetric has tracked 2,296 research outputs from this source. They receive a mean Attention Score of 4.2. This one has done well, scoring higher than 85% of its peers.
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We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.