| Title |
RNA interference and the vaccine effect of a subolesin homolog from the tick Rhipicephalus haemaphysaloides
|
|---|---|
| Published in |
Experimental and Applied Acarology, November 2015
|
| DOI | 10.1007/s10493-015-9987-z |
| Pubmed ID | |
| Authors |
Pengyun Lu, Yongzhi Zhou, Yingfang Yu, Jie Cao, Houshuang Zhang, Haiyan Gong, Guoqing Li, Jinlin Zhou |
| Abstract |
Subolesin is a well-characterized protective antigen in many ticks and, thus, it is potentially useful in the development of a broad-spectrum vaccine or an autocidal gene silencing strategy to control tick infestations. A subolesin homolog was cloned from the tick Rhipicephalus haemaphysaloides, which is widespread in China, by rapid amplification of complementary DNA (cDNA) ends. Its full-length cDNA was 1386 base pairs (bp), containing a 483 bp open reading frame with a predicted molecular mass of 18.7 kilodaltons and an isoelectric point of 9.26. The subolesin protein had a typical nuclear localization signal in its amino-terminus. The full-length cDNA of R. haemaphysaloides showed 52 and 80 % identities to those from Ixodes scapularis and R. microplus, respectively, whereas amino acid sequence alignments showed 80 and 97 % identities, respectively. Native subolesin was recognized in the unfed tick midgut by an antibody against recombinant subolesin. Transcriptional analysis showed that subolesin was expressed in the tick's four developmental stages and in all of the tissues examined, except for the synganglion. The pathogen Babesia microti induced the subolesin transcript by fourfold. Subolesin gene silencing by RNA interference significantly decreased the larval engorgement rate, the attachment rate and body weight of engorged nymphs, and the body weight and attachment and engorgement rates of adults, as well as the egg weight per female tick. Vaccinating mice and rabbits with recombinant subolesin induced a significant protective effect, resulting in a reduction of blood feeding and oviposition. These results encourage further studies of using subolesin to control tick infestations in China. |
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 26 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 19% |
| Student > Doctoral Student | 4 | 15% |
| Student > Master | 4 | 15% |
| Researcher | 3 | 12% |
| Student > Bachelor | 2 | 8% |
| Other | 2 | 8% |
| Unknown | 6 | 23% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 38% |
| Biochemistry, Genetics and Molecular Biology | 3 | 12% |
| Nursing and Health Professions | 1 | 4% |
| Veterinary Science and Veterinary Medicine | 1 | 4% |
| Psychology | 1 | 4% |
| Other | 3 | 12% |
| Unknown | 7 | 27% |
Attention Score in Context
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#21,186,729
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Outputs from Experimental and Applied Acarology
#718
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Outputs of similar age from Experimental and Applied Acarology
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