| Title |
Application of CRISPR/Cas9 technologies combined with iPSCs in the study and treatment of retinal degenerative diseases
|
|---|---|
| Published in |
Human Genetics, September 2018
|
| DOI | 10.1007/s00439-018-1933-9 |
| Pubmed ID | |
| Authors |
Bincui Cai, Shuo Sun, Zhiqing Li, Xiaomin Zhang, Yifeng Ke, Jin Yang, Xiaorong Li |
| Abstract |
Retinal degeneration diseases, such as age-related macular degeneration and retinitis pigmentosa, affect millions of people worldwide and are major causes of irreversible blindness. Effective treatments for retinal degeneration, including drug therapy, gene augmentation or transplantation approaches, have been widely investigated. Nevertheless, more research should be dedicated to therapeutic methods to improve future clinical treatments. Recently, with the rapid development of genome-editing technology, gene therapy has become a potentially effective treatment for retinal degeneration diseases. A clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system has been developed as a powerful genome-editing tool in ophthalmic studies. The CRISPR/Cas9 system has been widely applied in basic research to develop animal models and gene therapies in vivo. With the ability to self-renew and the potential to differentiate into different types of cells, induced pluripotent stem cells (iPSCs) have already been used as a promising tool for understanding disease pathophysiology and evaluating the effect of drug and gene therapeutics. iPSCs are also a cell source for autologous transplantation. In this review, we compared genome-editing strategies and highlighted the advantages and concerns of the CRISPR/Cas9 system. Moreover, the latest progress and applications of the CRISPR/Cas9 system and its combination with iPSCs for the treatment of retinal degenerative diseases are summarized. |
X Demographics
The data shown below were collected from the profiles of 12 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 17% |
| Unknown | 10 | 83% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 67% |
| Science communicators (journalists, bloggers, editors) | 2 | 17% |
| Scientists | 2 | 17% |
Mendeley readers
The data shown below were compiled from readership statistics for 82 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 82 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 23 | 28% |
| Researcher | 11 | 13% |
| Other | 6 | 7% |
| Student > Ph. D. Student | 5 | 6% |
| Student > Doctoral Student | 4 | 5% |
| Other | 13 | 16% |
| Unknown | 20 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 25 | 30% |
| Medicine and Dentistry | 16 | 20% |
| Agricultural and Biological Sciences | 4 | 5% |
| Business, Management and Accounting | 2 | 2% |
| Unspecified | 2 | 2% |
| Other | 10 | 12% |
| Unknown | 23 | 28% |
Attention Score in Context
This research output has an Altmetric Attention Score of 17. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 September 2022.
All research outputs
#1,909,870
of 23,213,531 outputs
Outputs from Human Genetics
#155
of 2,968 outputs
Outputs of similar age
#42,142
of 337,474 outputs
Outputs of similar age from Human Genetics
#2
of 27 outputs
Altmetric has tracked 23,213,531 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 91st percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,968 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.3. This one has done particularly well, scoring higher than 94% of its peers.
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We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.