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Functional metagenomic selection of ribulose 1, 5‐bisphosphate carboxylase/oxygenase from uncultivated bacteria

Overview of attention for article published in Environmental Microbiology, January 2016
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (87th percentile)
  • Good Attention Score compared to outputs of the same age and source (74th percentile)

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Title
Functional metagenomic selection of ribulose 1, 5‐bisphosphate carboxylase/oxygenase from uncultivated bacteria
Published in
Environmental Microbiology, January 2016
DOI 10.1111/1462-2920.13138
Pubmed ID
Authors

Vanessa A Varaljay, Sriram Satagopan, Justin A North, Brian Witte, Manuella N Dourado, Karthik Anantharaman, Mark A Arbing, Shelley Hoeft McCann, Ronald S Oremland, Jillian F Banfield, Kelly C Wrighton, F Robert Tabita

Abstract

Ribulose 1, 5-bisphosphate carboxylase/oxygenase (RubisCO) is a critical yet severely inefficient enzyme that catalyzes the fixation of virtually all of the carbon found on Earth. Here, we report a functional metagenomic selection that recovers physiologically-active RubisCO molecules directly from uncultivated and largely unknown members of natural microbial communities. Selection is based on CO2 -dependent growth in a host strain capable of expressing environmental DNA, precluding the need for pure cultures or screening of recombinant clones for enzymatic activity. Seventeen functional RubisCO-encoded sequences were selected using DNA extracted from soil and river autotrophic enrichments, a photosynthetic biofilm, and a subsurface groundwater aquifer. Notably, three related form II RubisCOs were recovered which share high sequence similarity with metagenomic scaffolds from uncultivated members of the Gallionellaceae family. One of the Gallionellaceae RubisCOs was purified and shown to possess CO2 /O2 specificity typical of form II enzymes. X-ray crystallography determined that this enzyme is a hexamer, only the second form II multimer ever solved and the first RubisCO structure obtained from an uncultivated bacterium. Functional metagenomic selection leverages natural biological diversity and billions of years of evolution inherent in environmental communities, providing a new window into the discovery of CO2 -fixing enzymes not previously characterized.

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X Demographics

The data shown below were collected from the profiles of 21 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 52 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 1 2%
United States 1 2%
Estonia 1 2%
Australia 1 2%
Unknown 48 92%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 10 19%
Student > Ph. D. Student 7 13%
Researcher 7 13%
Student > Master 7 13%
Student > Doctoral Student 5 10%
Other 8 15%
Unknown 8 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 17 33%
Biochemistry, Genetics and Molecular Biology 10 19%
Environmental Science 4 8%
Engineering 4 8%
Immunology and Microbiology 3 6%
Other 6 12%
Unknown 8 15%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 12. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 December 2017.
All research outputs
#2,944,023
of 25,381,384 outputs
Outputs from Environmental Microbiology
#699
of 4,602 outputs
Outputs of similar age
#48,553
of 405,787 outputs
Outputs of similar age from Environmental Microbiology
#28
of 106 outputs
Altmetric has tracked 25,381,384 research outputs across all sources so far. Compared to these this one has done well and is in the 88th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,602 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.6. This one has done well, scoring higher than 84% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 405,787 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 87% of its contemporaries.
We're also able to compare this research output to 106 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.