| Title |
Bunched and Madm Function Downstream of Tuberous Sclerosis Complex to Regulate the Growth of Intestinal Stem Cells in Drosophila
|
|---|---|
| Published in |
Stem Cell Reviews and Reports, September 2015
|
| DOI | 10.1007/s12015-015-9617-5 |
| Pubmed ID | |
| Authors |
Yingchao Nie, Qi Li, Alla Amcheslavsky, Juan Carlos Duhart, Alexey Veraksa, Hugo Stocker, Laurel A. Raftery, Y. Tony Ip |
| Abstract |
The Drosophila adult midgut contains intestinal stem cells that support homeostasis and repair. We show here that the leucine zipper protein Bunched and the adaptor protein Madm are novel regulators of intestinal stem cells. MARCM mutant clonal analysis and cell type specific RNAi revealed that Bunched and Madm were required within intestinal stem cells for proliferation. Transgenic expression of a tagged Bunched showed a cytoplasmic localization in midgut precursors, and the addition of a nuclear localization signal to Bunched reduced its function to cooperate with Madm to increase intestinal stem cell proliferation. Furthermore, the elevated cell growth and 4EBP phosphorylation phenotypes induced by loss of Tuberous Sclerosis Complex or overexpression of Rheb were suppressed by the loss of Bunched or Madm. Therefore, while the mammalian homolog of Bunched, TSC-22, is able to regulate transcription and suppress cancer cell proliferation, our data suggest the model that Bunched and Madm functionally interact with the TOR pathway in the cytoplasm to regulate the growth and subsequent division of intestinal stem cells. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 2 | 6% |
| France | 1 | 3% |
| Unknown | 31 | 91% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 13 | 38% |
| Student > Ph. D. Student | 6 | 18% |
| Student > Bachelor | 4 | 12% |
| Professor > Associate Professor | 2 | 6% |
| Student > Master | 2 | 6% |
| Other | 4 | 12% |
| Unknown | 3 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 11 | 32% |
| Biochemistry, Genetics and Molecular Biology | 10 | 29% |
| Neuroscience | 5 | 15% |
| Medicine and Dentistry | 2 | 6% |
| Immunology and Microbiology | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 3 | 9% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 December 2015.
All research outputs
#22,759,802
of 25,374,917 outputs
Outputs from Stem Cell Reviews and Reports
#914
of 1,036 outputs
Outputs of similar age
#237,726
of 277,052 outputs
Outputs of similar age from Stem Cell Reviews and Reports
#18
of 20 outputs
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