| Title |
A single chromosome assembly of Bacteroides fragilis strain BE1 from Illumina and MinION nanopore sequencing data
|
|---|---|
| Published in |
Giga Science, December 2015
|
| DOI | 10.1186/s13742-015-0101-6 |
| Pubmed ID | |
| Authors |
Judith Risse, Marian Thomson, Sheila Patrick, Garry Blakely, Georgios Koutsovoulos, Mark Blaxter, Mick Watson |
| Abstract |
Second and third generation sequencing technologies have revolutionised bacterial genomics. Short-read Illumina reads result in cheap but fragmented assemblies, whereas longer reads are more expensive but result in more complete genomes. The Oxford Nanopore MinION device is a revolutionary mobile sequencer that can produce thousands of long, single molecule reads. We sequenced Bacteroides fragilis strain BE1 using both the Illumina MiSeq and Oxford Nanopore MinION platforms. We were able to assemble a single chromosome of 5.18 Mb, with no gaps, using publicly available software and commodity computing hardware. We identified gene rearrangements and the state of invertible promoters in the strain. The single chromosome assembly of Bacteroides fragilis strain BE1 was achieved using only modest amounts of data, publicly available software and commodity computing hardware. This combination of technologies offers the possibility of ultra-cheap, high quality, finished bacterial genomes. |
X Demographics
The data shown below were collected from the profiles of 44 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 11 | 25% |
| United Kingdom | 7 | 16% |
| Spain | 4 | 9% |
| Germany | 2 | 5% |
| France | 2 | 5% |
| Hong Kong | 1 | 2% |
| Netherlands | 1 | 2% |
| Norway | 1 | 2% |
| Australia | 1 | 2% |
| Other | 0 | 0% |
| Unknown | 14 | 32% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 23 | 52% |
| Members of the public | 20 | 45% |
| Science communicators (journalists, bloggers, editors) | 1 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 174 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 1% |
| United States | 2 | 1% |
| Sweden | 1 | <1% |
| Czechia | 1 | <1% |
| Ghana | 1 | <1% |
| Canada | 1 | <1% |
| Netherlands | 1 | <1% |
| Japan | 1 | <1% |
| New Zealand | 1 | <1% |
| Other | 0 | 0% |
| Unknown | 163 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 41 | 24% |
| Student > Master | 30 | 17% |
| Student > Ph. D. Student | 29 | 17% |
| Student > Bachelor | 19 | 11% |
| Other | 8 | 5% |
| Other | 25 | 14% |
| Unknown | 22 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 69 | 40% |
| Biochemistry, Genetics and Molecular Biology | 34 | 20% |
| Computer Science | 12 | 7% |
| Immunology and Microbiology | 11 | 6% |
| Medicine and Dentistry | 9 | 5% |
| Other | 14 | 8% |
| Unknown | 25 | 14% |
Attention Score in Context
This research output has an Altmetric Attention Score of 45. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 June 2021.
All research outputs
#927,481
of 25,411,814 outputs
Outputs from Giga Science
#128
of 1,168 outputs
Outputs of similar age
#15,431
of 394,871 outputs
Outputs of similar age from Giga Science
#3
of 18 outputs
Altmetric has tracked 25,411,814 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 1,168 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 21.8. This one has done well, scoring higher than 89% of its peers.
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We're also able to compare this research output to 18 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 83% of its contemporaries.