| Title |
Isolation and Complete Genome Sequence of a Novel Marinobacter Phage B23
|
|---|---|
| Published in |
Current Microbiology, September 2018
|
| DOI | 10.1007/s00284-018-1568-z |
| Pubmed ID | |
| Authors |
Min Zhu, Min Wang, Yong Jiang, Siyuan You, Guihua Zhao, Yundan Liu, Qingwei Yang, Qian Liu, Zhaoyang Liu, Zheng Gong, Hongbing Shao |
| Abstract |
We used the double-agar layer method to isolate a novel Marinobacter marina bacteriophage, B23, from the surface water sample of the Bohai sea of China. There is some work to better understand the phage. The result of transmission electron microscopy revealed that B23 belongs to the family Siphoviridae with a head of 80 nm in diameter and a tail of 230 nm. Microbiological characterization evidenced that phage B23 is stable at the temperatures from - 25 to 60 °C, and showed vigorous vitality at pH between 4.0 and 12.0. One-step growth experiment showed that it had a longer latent period and higher lysis efficiency. Furthermore, the complete genome of B23 was sequenced and analyzed, which consists of a 35132 bp DNA with a G + C content of 59.8% and 50 putative open reading frames. The genome was divided into five parts, consisting of DNA replication and regulation, phage packaging, phage structure, host lysis and hypothetical protein. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 2 | 67% |
| Unknown | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 19% |
| Student > Bachelor | 4 | 19% |
| Student > Master | 3 | 14% |
| Researcher | 2 | 10% |
| Unknown | 8 | 38% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 3 | 14% |
| Environmental Science | 2 | 10% |
| Immunology and Microbiology | 2 | 10% |
| Agricultural and Biological Sciences | 2 | 10% |
| Veterinary Science and Veterinary Medicine | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 9 | 43% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 September 2018.
All research outputs
#17,990,045
of 23,103,436 outputs
Outputs from Current Microbiology
#1,591
of 2,436 outputs
Outputs of similar age
#241,916
of 337,432 outputs
Outputs of similar age from Current Microbiology
#16
of 26 outputs
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