| Title |
Effect of lipopolysaccharide on the hemocyte apoptosis of Eriocheir sinensis
|
|---|---|
| Published in |
Journal of Zhejiang University - Science B, December 2015
|
| DOI | 10.1631/jzus.b1500098 |
| Pubmed ID | |
| Authors |
Hai-sheng Xu, Sun-jian Lyu, Jie-hao Xu, Bin-jie Lu, Jing Zhao, Song Li, Yi-qun Li, Yu-yin Chen |
| Abstract |
In the present study, we investigated the possible toxicity mechanism of lipopolysaccharide (LPS) extracted from Gram-negative bacteria in Eriocheir sinensis hemocytes. Apoptotic hemocytes and reactive oxygen species (ROS) production induced by the LPS were monitored by the combination of flow cytometry and microscope observation. It was shown that LPS induced serious damage on the DNA and morphological changes in hemocytes, including cell shrinkage, fracture of nucleus membrane, margination, condensation and fragmentation of chromatin, and formation of apoptotic bodies indicating obvious hemocyte apoptosis. As compared with the control group, the apoptotic cell ratio increased to 30.61% and 39.01% after 1-h exposure and 57.72% and 75.01% after 2-h exposure to 1 and 10 μg/ml LPS, respectively (P<0.05). Significant outburst of ROS production was observed in LPS-treated hemocytes with approximately 176.6% of relative dichlorofluorescein mean fluorescence at 1-h exposure, followed by a drastic decline (P<0.05). These results indicated that LPS would induce oxidative stress on hemocytes from E. sinensis and cause ROS burst, DNA damage, and subsequently apoptosis. The process of ROS-mediated apoptosis might be one of the potential toxicity mechanisms of LPS on crustacean hemocytes. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 24% |
| Researcher | 3 | 18% |
| Student > Doctoral Student | 1 | 6% |
| Student > Master | 1 | 6% |
| Student > Postgraduate | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 7 | 41% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 4 | 24% |
| Biochemistry, Genetics and Molecular Biology | 3 | 18% |
| Business, Management and Accounting | 1 | 6% |
| Immunology and Microbiology | 1 | 6% |
| Unknown | 8 | 47% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 December 2015.
All research outputs
#23,154,082
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Outputs from Journal of Zhejiang University - Science B
#555
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#327,260
of 382,410 outputs
Outputs of similar age from Journal of Zhejiang University - Science B
#8
of 14 outputs
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