| Title |
Resistance Mechanism in a Terbinafine-Resistant Strain of Microsporum canis
|
|---|---|
| Published in |
Mycopathologia, January 2018
|
| DOI | 10.1007/s11046-018-0242-0 |
| Pubmed ID | |
| Authors |
Rui Kano, Yun-Hsia Hsiao, Hock Siew Han, Charles Chen, Atsuhiko Hasegawa, Hiroshi Kamata |
| Abstract |
To clarify the terbinafine (TRF) resistance mechanism in a TRF-resistant strain of Microsporum canis, the expression of the pleiotropic drug resistance (PDR1), multidrug resistance (MDR1), MDR2 and MDR4 genes were investigated by real-time quantitative PCR (RT-qPCR) analysis, given the known interaction of the corresponding proteins with antifungals and with the efflux blocker FK506. The expression of the PDR1, MDR1, MDR2 and MDR4 genes was 2-4 times higher in the TRF-resistant strain grown in the presence of 0.14 µg/mL of TRF than in TRF-susceptible strains cultured in the absence of TRF. The TRF-resistant strain exhibited MICs of > 32 µg/mL for TRF alone; this resistance was attenuated to an MIC of 8 µg/mL in the presence of FK506, indicating that the TRF inhibitory concentration index value was < 0.75. The additive effect of the efflux blocker FK506 on TRF resistance was detected in the TRF-resistant strain. These results indicated that the TRF resistance in this strain reflects overexpression of genes encoding ABC transporter proteins. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Switzerland | 1 | 50% |
| United Kingdom | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 33 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 6 | 18% |
| Researcher | 3 | 9% |
| Student > Ph. D. Student | 3 | 9% |
| Student > Postgraduate | 2 | 6% |
| Professor | 2 | 6% |
| Other | 5 | 15% |
| Unknown | 12 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 6 | 18% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 12% |
| Medicine and Dentistry | 4 | 12% |
| Veterinary Science and Veterinary Medicine | 2 | 6% |
| Biochemistry, Genetics and Molecular Biology | 1 | 3% |
| Other | 2 | 6% |
| Unknown | 14 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 September 2018.
All research outputs
#18,649,666
of 23,103,903 outputs
Outputs from Mycopathologia
#784
of 1,080 outputs
Outputs of similar age
#331,333
of 442,482 outputs
Outputs of similar age from Mycopathologia
#7
of 11 outputs
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