Title |
Packed Bed Bioreactor for the Isolation and Expansion of Placental-Derived Mesenchymal Stromal Cells
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Published in |
PLOS ONE, December 2015
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DOI | 10.1371/journal.pone.0144941 |
Pubmed ID | |
Authors |
Michael J. Osiecki, Thomas D. Michl, Betul Kul Babur, Mahboubeh Kabiri, Kerry Atkinson, William B. Lott, Hans J. Griesser, Michael R. Doran |
Abstract |
Large numbers of Mesenchymal stem/stromal cells (MSCs) are required for clinical relevant doses to treat a number of diseases. To economically manufacture these MSCs, an automated bioreactor system will be required. Herein we describe the development of a scalable closed-system, packed bed bioreactor suitable for large-scale MSCs expansion. The packed bed was formed from fused polystyrene pellets that were air plasma treated to endow them with a surface chemistry similar to traditional tissue culture plastic. The packed bed was encased within a gas permeable shell to decouple the medium nutrient supply and gas exchange. This enabled a significant reduction in medium flow rates, thus reducing shear and even facilitating single pass medium exchange. The system was optimised in a small-scale bioreactor format (160 cm2) with murine-derived green fluorescent protein-expressing MSCs, and then scaled-up to a 2800 cm2 format. We demonstrated that placental derived MSCs could be isolated directly within the bioreactor and subsequently expanded. Our results demonstrate that the closed system large-scale packed bed bioreactor is an effective and scalable tool for large-scale isolation and expansion of MSCs. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 1% |
United States | 1 | 1% |
Unknown | 74 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 16 | 21% |
Researcher | 16 | 21% |
Student > Master | 15 | 20% |
Student > Doctoral Student | 4 | 5% |
Other | 4 | 5% |
Other | 10 | 13% |
Unknown | 11 | 14% |
Readers by discipline | Count | As % |
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Engineering | 13 | 17% |
Agricultural and Biological Sciences | 12 | 16% |
Medicine and Dentistry | 9 | 12% |
Chemical Engineering | 7 | 9% |
Biochemistry, Genetics and Molecular Biology | 7 | 9% |
Other | 10 | 13% |
Unknown | 18 | 24% |