| Title |
Developing a 3D intestinal epithelium model for livestock species
|
|---|---|
| Published in |
Cell and Tissue Research, September 2018
|
| DOI | 10.1007/s00441-018-2924-9 |
| Pubmed ID | |
| Authors |
Hayley Derricott, Lisa Luu, Wai Yee Fong, Catherine S. Hartley, Luke J. Johnston, Stuart D. Armstrong, Nadine Randle, Carrie A. Duckworth, Barry J. Campbell, Jonathan M. Wastling, Janine L. Coombes |
| Abstract |
The in vitro 3D culture of intestinal epithelium is a valuable resource in the study of its function. Organoid culture exploits stem cells' ability to regenerate and produce differentiated epithelium. Intestinal organoid models from rodent or human tissue are widely available whereas large animal models are not. Livestock enteric and zoonotic diseases elicit significant morbidity and mortality in animal and human populations. Therefore, livestock species-specific models may offer novel insights into host-pathogen interactions and disease responses. Bovine and porcine jejunum were obtained from an abattoir and their intestinal crypts isolated, suspended in Matrigel, cultured, cryopreserved and resuscitated. 'Rounding' of crypts occurred followed by budding and then enlargement of the organoids. Epithelial cells were characterised using immunofluorescent staining and confocal microscopy. Organoids were successfully infected with Toxoplasma gondii or Salmonella typhimurium. This 3D organoid model offers a long-term, renewable resource for investigating species-specific intestinal infections with a variety of pathogens. |
X Demographics
The data shown below were collected from the profiles of 12 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 6 | 50% |
| Italy | 1 | 8% |
| Canada | 1 | 8% |
| United States | 1 | 8% |
| Sweden | 1 | 8% |
| Unknown | 2 | 17% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 67% |
| Scientists | 3 | 25% |
| Science communicators (journalists, bloggers, editors) | 1 | 8% |
Mendeley readers
The data shown below were compiled from readership statistics for 81 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 81 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 11 | 14% |
| Researcher | 11 | 14% |
| Student > Bachelor | 9 | 11% |
| Student > Master | 7 | 9% |
| Other | 4 | 5% |
| Other | 11 | 14% |
| Unknown | 28 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 14 | 17% |
| Agricultural and Biological Sciences | 11 | 14% |
| Immunology and Microbiology | 9 | 11% |
| Veterinary Science and Veterinary Medicine | 6 | 7% |
| Nursing and Health Professions | 3 | 4% |
| Other | 8 | 10% |
| Unknown | 30 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2019.
All research outputs
#4,130,010
of 23,839,820 outputs
Outputs from Cell and Tissue Research
#176
of 2,279 outputs
Outputs of similar age
#79,115
of 343,251 outputs
Outputs of similar age from Cell and Tissue Research
#2
of 27 outputs
Altmetric has tracked 23,839,820 research outputs across all sources so far. Compared to these this one has done well and is in the 82nd percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,279 research outputs from this source. They receive a mean Attention Score of 3.3. This one has done particularly well, scoring higher than 92% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 343,251 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 76% of its contemporaries.
We're also able to compare this research output to 27 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 92% of its contemporaries.