| Title |
An integrated chip-mass spectrometry and epifluorescence approach for online monitoring of bioactive metabolites from incubated Actinobacteria in picoliter droplets
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, September 2018
|
| DOI | 10.1007/s00216-018-1383-1 |
| Pubmed ID | |
| Authors |
Konstantin Wink, Lisa Mahler, Julia R. Beulig, Sebastian K. Piendl, Martin Roth, Detlev Belder |
| Abstract |
We present a lab-on-a-chip approach for the analysis of secondary metabolites produced in microfluidic droplets by simultaneous epifluorescence microscopy and electrospray ionization mass spectrometry (ESI-MS). The approach includes encapsulation and long-term off-chip incubation of microbes in surfactant-stabilized droplets followed by a transfer of droplets into a microfluidic chip for subsequent analysis. Before the reinjected droplets are spaced and electrosprayed from an integrated emitter into a mass spectrometer, the presence of fluorescent marker molecules is monitored nearly simultaneously with a custom-made portable epifluorescence microscope. This combined fluorescence and MS-detection setup allows the analysis of metabolites and fluorescent labels in a complex biological matrix at a single droplet level. Using hyphae of Streptomyces griseus, encapsulated in microfluidic droplets of ~ 200 picoliter as a model system, we show the detection of in situ produced streptomycin by ESI-MS and the feasibility of detecting fluorophores inside droplets shortly before they are electrosprayed. The presented method expands the analytical toolbox for the discovery of bioactive metabolites such as novel antibiotics, produced by microorganisms. |
X Demographics
The data shown below were collected from the profiles of 7 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 14% |
| Germany | 1 | 14% |
| United Kingdom | 1 | 14% |
| Unknown | 4 | 57% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 57% |
| Scientists | 2 | 29% |
| Science communicators (journalists, bloggers, editors) | 1 | 14% |
Mendeley readers
The data shown below were compiled from readership statistics for 58 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 58 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 19 | 33% |
| Student > Master | 7 | 12% |
| Student > Doctoral Student | 5 | 9% |
| Researcher | 5 | 9% |
| Student > Bachelor | 2 | 3% |
| Other | 5 | 9% |
| Unknown | 15 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 14 | 24% |
| Biochemistry, Genetics and Molecular Biology | 7 | 12% |
| Chemical Engineering | 4 | 7% |
| Engineering | 4 | 7% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 5% |
| Other | 9 | 16% |
| Unknown | 17 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 November 2018.
All research outputs
#14,608,799
of 25,385,509 outputs
Outputs from Analytical & Bioanalytical Chemistry
#4,352
of 9,619 outputs
Outputs of similar age
#173,136
of 352,583 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#30
of 146 outputs
Altmetric has tracked 25,385,509 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,619 research outputs from this source. They receive a mean Attention Score of 3.1. This one has gotten more attention than average, scoring higher than 54% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 352,583 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 146 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 79% of its contemporaries.