| Title |
Recent advances in CRISPR/Cas9 mediated genome editing in Bacillus subtilis
|
|---|---|
| Published in |
World Journal of Microbiology and Biotechnology, September 2018
|
| DOI | 10.1007/s11274-018-2537-1 |
| Pubmed ID | |
| Authors |
Kun-Qiang Hong, Ding-Yu Liu, Tao Chen, Zhi-Wen Wang |
| Abstract |
Genome editing using engineered nucleases has rapidly transformed from a niche technology to a mainstream method used in various host cells. Its widespread adoption has been largely developed by the emergence of the clustered regularly interspaced short palindromic repeats (CRISPR) system, which uses an easily customizable specificity RNA-guided DNA endonuclease, such as Cas9. Recently, CRISPR/Cas9 mediated genome engineering has been widely applied to model organisms, including Bacillus subtilis, enabling facile, rapid high-fidelity modification of endogenous native genes. Here, we reviewed the recent progress in B. subtilis gene editing using CRISPR/Cas9 based tools, and highlighted state-of-the-art strategies for design of CRISPR/Cas9 system. Finally, future perspectives on the use of CRISPR/Cas9 genome engineering for sequence-specific genome editing in B. subtilis are provided. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Argentina | 1 | 20% |
| Netherlands | 1 | 20% |
| Unknown | 3 | 60% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 80% |
| Scientists | 1 | 20% |
Mendeley readers
The data shown below were compiled from readership statistics for 94 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 94 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 20 | 21% |
| Researcher | 11 | 12% |
| Student > Master | 10 | 11% |
| Student > Bachelor | 8 | 9% |
| Student > Doctoral Student | 4 | 4% |
| Other | 11 | 12% |
| Unknown | 30 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 30 | 32% |
| Agricultural and Biological Sciences | 15 | 16% |
| Immunology and Microbiology | 5 | 5% |
| Medicine and Dentistry | 3 | 3% |
| Engineering | 2 | 2% |
| Other | 3 | 3% |
| Unknown | 36 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2020.
All research outputs
#6,550,591
of 23,911,072 outputs
Outputs from World Journal of Microbiology and Biotechnology
#255
of 1,757 outputs
Outputs of similar age
#113,242
of 345,250 outputs
Outputs of similar age from World Journal of Microbiology and Biotechnology
#6
of 31 outputs
Altmetric has tracked 23,911,072 research outputs across all sources so far. This one has received more attention than most of these and is in the 72nd percentile.
So far Altmetric has tracked 1,757 research outputs from this source. They receive a mean Attention Score of 2.9. This one has done well, scoring higher than 85% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 345,250 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.
We're also able to compare this research output to 31 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 80% of its contemporaries.