| Title |
First identification of Sarcocystis hominis in Iranian traditional hamburger
|
|---|---|
| Published in |
Journal of Parasitic Diseases, January 2014
|
| DOI | 10.1007/s12639-014-0425-7 |
| Pubmed ID | |
| Authors |
M. Moghaddam Ahmadi, B. Hajimohammadi, G. Eslami, A. Oryan, S. A. Yasini Ardakani, A. Zohourtabar, S. Zare |
| Abstract |
Zoonotic concerns of cattle sarcocystosis are of importance, because humans are the final host for Sarcocystis hominis. Therefore the meat products containing beef may encompass sarcocysts which endanger food safety. In this study, we described the first report of molecular identification of S. hominis in Iranian traditional hamburgers using PCR-RFLP. Throughout a pilot research that was carried out to setup a molecular approach to identify the Sarcocystis spp., using PCR-RFLP, a sample of raw Iranian traditional hamburger was purchased from a street food seller located in Yazd, central Iran in May 2013. DNA extraction was done, by salting out method; briefly, the sample was lysed with NET buffer. The DNA purification and precipitation was then performed. Amplicon and digestion results were analyzed, using gel agarose electrophoresis. The results showed a PCR product with 926 bp in length after amplification and 376 and 550 bp in length after digestion. This product was identified as S. hominis. To the best of our knowledge, this is the first report of S. hominis infection in Iranian hamburger. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 5 | 24% |
| Other | 3 | 14% |
| Professor | 3 | 14% |
| Student > Bachelor | 2 | 10% |
| Student > Ph. D. Student | 2 | 10% |
| Other | 3 | 14% |
| Unknown | 3 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Veterinary Science and Veterinary Medicine | 5 | 24% |
| Biochemistry, Genetics and Molecular Biology | 3 | 14% |
| Medicine and Dentistry | 2 | 10% |
| Agricultural and Biological Sciences | 2 | 10% |
| Environmental Science | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 6 | 29% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 December 2015.
All research outputs
#20,299,108
of 22,836,570 outputs
Outputs from Journal of Parasitic Diseases
#276
of 430 outputs
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#265,990
of 307,328 outputs
Outputs of similar age from Journal of Parasitic Diseases
#12
of 28 outputs
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