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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Structurally responsive oligonucleotide-based single-probe lateral-flow test for detection of miRNA-21 mimics
|
|---|---|
| Published in |
Analytical & Bioanalytical Chemistry, December 2015
|
| DOI | 10.1007/s00216-015-9250-9 |
| Pubmed ID | |
| Authors |
Kamalodin Kor, Anthony P. F. Turner, Kobra Zarei, Morteza Atabati, Valerio Beni, Wing Cheung Mak |
| Abstract |
A single-probe strip test for the rapid and sensitive detection of miRNA-21 mimics is reported herein. Highly specific structurally responsive bi-functional, thiol and biotin, DNA/LNA oligonucleotide probes (molecular beacons-MB) were designed and conjugated with gold nanoparticles (AuNPs) (i.e. biotin-MB-AuNPs). The proposed design had the ability to modulate the accessibility of the biotin group as a function of the presence of a miRNA target allowing the interaction of the boilable with the streptavidin test zone only in the presence of the miRNA-21 mimics. For quantitative evaluation, images of the strip tests were recorded using a flatbed scanner (Epson Perfection V370 Photo). The colour intensities of the test zones of the strip tests were analysed with the ImageJ software (Scion Corp., USA) and quantified as a function of pixel intensity. The response of the strip test was linear over the range 0.5 to 20 nM miRNA-21 (limit of detection of 115 pM) and showed good reproducibility (intra and inter CVs below 8 %); furthermore, the assay was shown to be highly selective, discriminating other interference miRNAs mimics (e.g. miRNA-221 and miRNA-205). Finally, the proposed strip test was used for detection of miRNA-21 mimics in spiked serum samples, demonstrating its potential for point-of-care clinical applications. Main advantages of the single-probe strip test design are its versatility, simplicity and robustness, which can be easily extended to other miRNA targets by tuning the sequence of the single probe. Furthermore, the use of the structurally responsive single probe is particularly relevant in the case of short-length targets, such as miRNA, whereas a conventional sandwich approach might require a careful control of assay conditions such as hybridization temperature and salt concentration. Graphical Abstract Pictorial representation of the working principle of the proposed structurally responsive oligonucleotide-based single-probe lateral-flow test and example of the test response as a function of increasing concentration of target analyte. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 42 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 9 | 21% |
| Student > Bachelor | 7 | 17% |
| Student > Ph. D. Student | 5 | 12% |
| Student > Master | 4 | 10% |
| Professor > Associate Professor | 3 | 7% |
| Other | 4 | 10% |
| Unknown | 10 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 19% |
| Chemistry | 7 | 17% |
| Agricultural and Biological Sciences | 4 | 10% |
| Engineering | 3 | 7% |
| Chemical Engineering | 2 | 5% |
| Other | 7 | 17% |
| Unknown | 11 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 January 2016.
All research outputs
#19,944,091
of 25,373,627 outputs
Outputs from Analytical & Bioanalytical Chemistry
#6,060
of 9,619 outputs
Outputs of similar age
#275,914
of 396,498 outputs
Outputs of similar age from Analytical & Bioanalytical Chemistry
#48
of 91 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 18th percentile – i.e., 18% of other outputs scored the same or lower than it.
So far Altmetric has tracked 9,619 research outputs from this source. They receive a mean Attention Score of 3.1. This one is in the 31st percentile – i.e., 31% of its peers scored the same or lower than it.
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We're also able to compare this research output to 91 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.