| Title |
Methylation patterns of Vegfb promoter are associated with gene and protein expression levels: the effects of dietary fatty acids
|
|---|---|
| Published in |
European Journal of Nutrition, December 2015
|
| DOI | 10.1007/s00394-015-1115-7 |
| Pubmed ID | |
| Authors |
Roberto Monastero, Sara García-Serrano, Ana Lago-Sampedro, Francisca Rodríguez-Pacheco, Natalia Colomo, Sonsoles Morcillo, Gracia M. Martín-Nuñez, Juan M. Gomez-Zumaquero, Eduardo García-Fuentes, Federico Soriguer, Gemma Rojo-Martínez, Eva García-Escobar |
| Abstract |
We have investigated the epigenetic regulation by dietary fatty acids of Vegfb levels in rats' white adipose tissue and 3T3-L1 cells. A group of rats were assigned to three diets, each one with a different composition of saturated, monounsaturated and polyunsaturated fatty acids. Samples of white adipose tissues were taken for the methylation and expression studies. Additionally, 3T3-L1 cells were treated with palmitic, oleic, and linoleic fatty acids. After treatment, cells were harvested and genetic material was extracted for the analysis of Vegfb levels. We report evidence of changes in the methylation levels of the CpG island at the Vegfb promoter and in the Vegfb expression levels in vivo and in vitro by dietary fatty acid, with the main contribution of the linoleic fatty acid. Vegfb promoter methylation levels were closely related to the Vegfb gene expression. According to our results, the regulation of Vegfb gene expression by dietary fatty acids may be mediated, at least in part, by epigenetic modifications on Vegfb promoter methylation. Considering the deep association between angiogenesis and tissue growth, we suggest the nutriepigenetic regulation of Vegfb as a key target in the control of the adipose tissue expansion. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 2% |
| Spain | 1 | 2% |
| Unknown | 39 | 95% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 17% |
| Student > Ph. D. Student | 5 | 12% |
| Student > Bachelor | 4 | 10% |
| Student > Master | 4 | 10% |
| Professor > Associate Professor | 3 | 7% |
| Other | 7 | 17% |
| Unknown | 11 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 20% |
| Medicine and Dentistry | 7 | 17% |
| Nursing and Health Professions | 5 | 12% |
| Agricultural and Biological Sciences | 4 | 10% |
| Sports and Recreations | 1 | 2% |
| Other | 3 | 7% |
| Unknown | 13 | 32% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 December 2015.
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#20,299,108
of 22,836,570 outputs
Outputs from European Journal of Nutrition
#2,130
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Outputs of similar age
#328,472
of 391,336 outputs
Outputs of similar age from European Journal of Nutrition
#53
of 57 outputs
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