| Title |
Targeted transcriptional repression using a chimeric TALE-SRDX repressor protein
|
|---|---|
| Published in |
Plant Molecular Biology, December 2011
|
| DOI | 10.1007/s11103-011-9866-x |
| Pubmed ID | |
| Authors |
Magdy M. Mahfouz, Lixin Li, Marek Piatek, Xiaoyun Fang, Hicham Mansour, Dhinoth K. Bangarusamy, Jian-Kang Zhu |
| Abstract |
Transcriptional activator-like effectors (TALEs) are proteins secreted by Xanthomonas bacteria when they infect plants. TALEs contain a modular DNA binding domain that can be easily engineered to bind any sequence of interest, and have been used to provide user-selected DNA-binding modules to generate chimeric nucleases and transcriptional activators in mammalian cells and plants. Here we report the use of TALEs to generate chimeric sequence-specific transcriptional repressors. The dHax3 TALE was used as a scaffold to provide a DNA-binding module fused to the EAR-repression domain (SRDX) to generate a chimeric repressor that targets the RD29A promoter. The dHax3.SRDX protein efficiently repressed the transcription of the RD29A::LUC transgene and endogenous RD29A gene in Arabidopsis. Genome wide expression profiling showed that the chimeric repressor also inhibited the expression of several other genes that contain the designer TALE-target sequence in their promoters. Our data suggest that TALEs can be used to generate chimeric repressors to specifically repress the transcription of genes of interest in plants. This sequence-specific transcriptional repression by direct on promoter effector technology is a powerful tool for functional genomics studies and biotechnological applications. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 197 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 4 | 2% |
| United States | 3 | 2% |
| Germany | 2 | 1% |
| Brazil | 2 | 1% |
| Slovenia | 1 | <1% |
| Italy | 1 | <1% |
| Japan | 1 | <1% |
| Mexico | 1 | <1% |
| Unknown | 182 | 92% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 47 | 24% |
| Researcher | 42 | 21% |
| Student > Master | 22 | 11% |
| Student > Bachelor | 20 | 10% |
| Student > Postgraduate | 9 | 5% |
| Other | 24 | 12% |
| Unknown | 33 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 109 | 55% |
| Biochemistry, Genetics and Molecular Biology | 32 | 16% |
| Chemistry | 4 | 2% |
| Engineering | 3 | 2% |
| Medicine and Dentistry | 3 | 2% |
| Other | 8 | 4% |
| Unknown | 38 | 19% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 February 2024.
All research outputs
#3,325,463
of 23,509,253 outputs
Outputs from Plant Molecular Biology
#125
of 2,859 outputs
Outputs of similar age
#26,757
of 246,085 outputs
Outputs of similar age from Plant Molecular Biology
#1
of 14 outputs
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So far Altmetric has tracked 2,859 research outputs from this source. They receive a mean Attention Score of 4.3. This one has done particularly well, scoring higher than 95% of its peers.
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