| Title |
Quantitative target analysis and kinetic profiling of acyl-CoAs reveal the rate-limiting step in cyanobacterial 1-butanol production
|
|---|---|
| Published in |
Metabolomics, January 2016
|
| DOI | 10.1007/s11306-015-0940-2 |
| Pubmed ID | |
| Authors |
Shingo Noguchi, Sastia P. Putri, Ethan I. Lan, Walter A. Laviña, Yudai Dempo, Takeshi Bamba, James C. Liao, Eiichiro Fukusaki |
| Abstract |
Cyanobacterial 1-butanol production is an important model system for direct conversion of CO2 to fuels and chemicals. Metabolically-engineered cyanobacteria introduced with a heterologous Coenzyme A (CoA)-dependent pathway modified from Clostridium species can convert atmospheric CO2 into 1-butanol. Efforts to optimize the 1-butanol pathway in Synechococcus elongatus PCC 7942 have focused on the improvement of the CoA-dependent pathway thus, probing the in vivo metabolic state of the CoA-dependent pathway is essential for identifying its limiting steps. In this study, we performed quantitative target analysis and kinetic profiling of acyl-CoAs in the CoA-dependent pathway by reversed phase ion-pair liquid chromatography-triple quadrupole mass spectrometry. Using (13)C-labelled cyanobacterial cell extract as internal standard, measurement of the intracellular concentration of acyl-CoAs revealed that the reductive reaction of butanoyl-CoA to butanal is a possible rate-limiting step. In addition, improvement of the butanoyl-CoA to butanal reaction resulted in an increased rate of acetyl-CoA synthesis by possibly compensating for the limitation of free CoA species. We inferred that the efficient recycling of free CoA played a key role in enhancing the conversion of pyruvate to acetyl-CoA. |
X Demographics
The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 25% |
| Unknown | 3 | 75% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 75% |
| Science communicators (journalists, bloggers, editors) | 1 | 25% |
Mendeley readers
The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 1% |
| Spain | 1 | 1% |
| Sweden | 1 | 1% |
| Unknown | 68 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 24% |
| Researcher | 14 | 20% |
| Student > Bachelor | 6 | 8% |
| Student > Doctoral Student | 5 | 7% |
| Student > Master | 5 | 7% |
| Other | 10 | 14% |
| Unknown | 14 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 27 | 38% |
| Biochemistry, Genetics and Molecular Biology | 11 | 15% |
| Engineering | 7 | 10% |
| Chemistry | 4 | 6% |
| Chemical Engineering | 2 | 3% |
| Other | 5 | 7% |
| Unknown | 15 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 March 2016.
All research outputs
#14,366,444
of 24,143,470 outputs
Outputs from Metabolomics
#701
of 1,342 outputs
Outputs of similar age
#197,877
of 401,899 outputs
Outputs of similar age from Metabolomics
#22
of 36 outputs
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