| Title |
Prokaryotic diversity in a Tunisian hypersaline lake, Chott El Jerid
|
|---|---|
| Published in |
Extremophiles, January 2016
|
| DOI | 10.1007/s00792-015-0805-7 |
| Pubmed ID | |
| Authors |
Manel Ben Abdallah, Fatma Karray, Najla Mhiri, Nan Mei, Marianne Quéméneur, Jean-Luc Cayol, Gaël Erauso, Jean-Luc Tholozan, Didier Alazard, Sami Sayadi |
| Abstract |
Prokaryotic diversity was investigated in a Tunisian salt lake, Chott El Jerid, by quantitative real-time PCR, denaturing gradient gel electrophoresis (DGGE) fingerprinting methods targeting the 16S rRNA gene and culture-dependent methods. Two different samples S1-10 and S2-10 were taken from under the salt crust of Chott El Jerid in the dry season. DGGE analysis revealed that bacterial sequences were related to Firmicutes, Proteobacteria, unclassified bacteria, and Deinococcus-Thermus phyla. Anaerobic fermentative and sulfate-reducing bacteria were also detected in this ecosystem. Within the domain archaea, all sequences were affiliated to Euryarchaeota phylum. Quantitative real-time PCR showed that 16S rRNA gene copy numbers of bacteria was 5 × 10(6) DNA copies g(-1) whereas archaea varied between 5 × 10(5) and 10(6) DNA copies g(-1) in these samples. Eight anaerobic halophilic fermentative bacterial strains were isolated and affiliated with the species Halanaerobium alcaliphilum, Halanaerobium saccharolyticum, and Sporohalobacter salinus. These data showed an abundant and diverse microbial community detected in the hypersaline thalassohaline environment of Chott El Jerid. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 31 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 29% |
| Researcher | 5 | 16% |
| Student > Doctoral Student | 3 | 10% |
| Professor | 2 | 6% |
| Student > Bachelor | 1 | 3% |
| Other | 3 | 10% |
| Unknown | 8 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 26% |
| Biochemistry, Genetics and Molecular Biology | 4 | 13% |
| Environmental Science | 3 | 10% |
| Immunology and Microbiology | 2 | 6% |
| Engineering | 2 | 6% |
| Other | 3 | 10% |
| Unknown | 9 | 29% |
Attention Score in Context
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All research outputs
#18,909,315
of 24,093,053 outputs
Outputs from Extremophiles
#611
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#275,787
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Outputs of similar age from Extremophiles
#5
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