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Identification of a Hemagglutinin from Gallibacterium anatis

Overview of attention for article published in Current Microbiology, January 2016
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Title
Identification of a Hemagglutinin from Gallibacterium anatis
Published in
Current Microbiology, January 2016
DOI 10.1007/s00284-015-0969-5
Pubmed ID
Authors

J. F. Montes-García, S. Vaca, C. Vazquez-Cruz, E. Soriano-Vargas, F. Aguilar-Romero, P. J. Blackall, E. Negrete-Abascal

Abstract

Gallibacterium anatis has the ability to hemagglutinate rabbit erythrocytes; however, no bacterial component has yet been associated with this function. In the present work, a protein of approximately 65 kDa with hemagglutinating activity for glutaraldehyde-fixed chicken erythrocytes was purified by ion interchange chromatography from G. anatis F149(T) secreted proteins. The protein was recognized by a rabbit polyclonal serum against a hemagglutinin from Avibacterium paragallinarum. The 65 kDa purified protein presented identity with a G. anatis filamentous hemagglutinin by mass spectrometric analysis. As well, the bacterial surface of G. anatis was labeled by immune gold assays using a polyclonal serum against the 65-kDa protein. A similar protein was recognized in four other G. anatis strains by immunoblots using the same antiserum. The protein binds sheep or pig biotinylated fibrinogen, suggesting an interaction with basement membrane eukaryotic cells components, and the protein is present in G. anatis biofilms. Overall, the results suggest that the 65 kDa hemagglutinin is a common antigen and a potential virulence factor in G. anatis.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 16 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 16 100%

Demographic breakdown

Readers by professional status Count As %
Student > Postgraduate 3 19%
Researcher 3 19%
Professor 2 13%
Student > Ph. D. Student 2 13%
Student > Doctoral Student 1 6%
Other 2 13%
Unknown 3 19%
Readers by discipline Count As %
Veterinary Science and Veterinary Medicine 3 19%
Agricultural and Biological Sciences 3 19%
Immunology and Microbiology 3 19%
Biochemistry, Genetics and Molecular Biology 2 13%
Nursing and Health Professions 1 6%
Other 1 6%
Unknown 3 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 06 January 2016.
All research outputs
#20,300,248
of 22,837,982 outputs
Outputs from Current Microbiology
#1,919
of 2,415 outputs
Outputs of similar age
#330,211
of 393,291 outputs
Outputs of similar age from Current Microbiology
#28
of 46 outputs
Altmetric has tracked 22,837,982 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,415 research outputs from this source. They receive a mean Attention Score of 3.2. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.