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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Reprogramming triggers endogenous L1 and Alu retrotransposition in human induced pluripotent stem cells
|
|---|---|
| Published in |
Nature Communications, January 2016
|
| DOI | 10.1038/ncomms10286 |
| Pubmed ID | |
| Authors |
Sabine Klawitter, Nina V. Fuchs, Kyle R. Upton, Martin Muñoz-Lopez, Ruchi Shukla, Jichang Wang, Marta Garcia-Cañadas, Cesar Lopez-Ruiz, Daniel J. Gerhardt, Attila Sebe, Ivana Grabundzija, Sylvia Merkert, Patricia Gerdes, J. Andres Pulgarin, Anja Bock, Ulrike Held, Anett Witthuhn, Alexandra Haase, Balázs Sarkadi, Johannes Löwer, Ernst J. Wolvetang, Ulrich Martin, Zoltán Ivics, Zsuzsanna Izsvák, Jose L. Garcia-Perez, Geoffrey J. Faulkner, Gerald G. Schumann |
| Abstract |
Human induced pluripotent stem cells (hiPSCs) are capable of unlimited proliferation and can differentiate in vitro to generate derivatives of the three primary germ layers. Genetic and epigenetic abnormalities have been reported by Wissing and colleagues to occur during hiPSC derivation, including mobilization of engineered LINE-1 (L1) retrotransposons. However, incidence and functional impact of endogenous retrotransposition in hiPSCs are yet to be established. Here we apply retrotransposon capture sequencing to eight hiPSC lines and three human embryonic stem cell (hESC) lines, revealing endogenous L1, Alu and SINE-VNTR-Alu (SVA) mobilization during reprogramming and pluripotent stem cell cultivation. Surprisingly, 4/7 de novo L1 insertions are full length and 6/11 retrotransposition events occurred in protein-coding genes expressed in pluripotent stem cells. We further demonstrate that an intronic L1 insertion in the CADPS2 gene is acquired during hiPSC cultivation and disrupts CADPS2 expression. These experiments elucidate endogenous retrotransposition, and its potential consequences, in hiPSCs and hESCs. |
X Demographics
The data shown below were collected from the profiles of 25 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 7 | 28% |
| Australia | 3 | 12% |
| France | 3 | 12% |
| United Kingdom | 1 | 4% |
| Germany | 1 | 4% |
| Spain | 1 | 4% |
| Unknown | 9 | 36% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 13 | 52% |
| Scientists | 10 | 40% |
| Practitioners (doctors, other healthcare professionals) | 1 | 4% |
| Science communicators (journalists, bloggers, editors) | 1 | 4% |
Mendeley readers
The data shown below were compiled from readership statistics for 184 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 3 | 2% |
| United States | 2 | 1% |
| Japan | 2 | 1% |
| Brazil | 1 | <1% |
| Germany | 1 | <1% |
| France | 1 | <1% |
| Norway | 1 | <1% |
| Unknown | 173 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 42 | 23% |
| Student > Ph. D. Student | 38 | 21% |
| Student > Master | 20 | 11% |
| Student > Bachelor | 16 | 9% |
| Student > Postgraduate | 9 | 5% |
| Other | 28 | 15% |
| Unknown | 31 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 65 | 35% |
| Agricultural and Biological Sciences | 64 | 35% |
| Medicine and Dentistry | 13 | 7% |
| Neuroscience | 3 | 2% |
| Immunology and Microbiology | 2 | 1% |
| Other | 3 | 2% |
| Unknown | 34 | 18% |
Attention Score in Context
This research output has an Altmetric Attention Score of 34. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 May 2023.
All research outputs
#1,129,075
of 24,792,414 outputs
Outputs from Nature Communications
#17,563
of 53,949 outputs
Outputs of similar age
#20,012
of 404,946 outputs
Outputs of similar age from Nature Communications
#250
of 749 outputs
Altmetric has tracked 24,792,414 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 95th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 53,949 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 56.0. This one has gotten more attention than average, scoring higher than 67% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 404,946 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 95% of its contemporaries.
We're also able to compare this research output to 749 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.