| Title |
Transcriptome Profiling of Wild Arachis from Water-Limited Environments Uncovers Drought Tolerance Candidate Genes
|
|---|---|
| Published in |
Plant Molecular Biology Reporter, April 2015
|
| DOI | 10.1007/s11105-015-0882-x |
| Pubmed ID | |
| Authors |
Ana C. M. Brasileiro, Carolina V. Morgante, Ana C. G. Araujo, Soraya C. M. Leal-Bertioli, Amanda K. Silva, Andressa C. Q. Martins, Christina C. Vinson, Candice M. R. Santos, Orzenil Bonfim, Roberto C. Togawa, Mario A. P. Saraiva, David J. Bertioli, Patricia M. Guimaraes |
| Abstract |
Peanut (Arachis hypogaea L.) is an important legume cultivated mostly in drought-prone areas where its productivity can be limited by water scarcity. The development of more drought-tolerant varieties is, therefore, a priority for peanut breeding programs worldwide. In contrast to cultivated peanut, wild relatives have a broader genetic diversity and constitute a rich source of resistance/tolerance alleles to biotic and abiotic stresses. The present study takes advantage of this diversity to identify drought-responsive genes by analyzing the expression profile of two wild species, Arachis duranensis and Arachis magna (AA and BB genomes, respectively), in response to progressive water deficit in soil. Data analysis from leaves and roots of A. duranensis (454 sequencing) and A. magna (suppression subtractive hybridization (SSH)) stressed and control complementary DNA (cDNA) libraries revealed several differentially expressed genes in silico, and 44 of them were selected for further validation by quantitative RT-PCR (qRT-PCR). This allowed the identification of drought-responsive candidate genes, such as Expansin, Nitrilase, NAC, and bZIP transcription factors, displaying significant levels of differential expression during stress imposition in both species. This is the first report on identification of differentially expressed genes under drought stress and recovery in wild Arachis species. The generated transcriptome data, besides being a valuable resource for gene discovery, will allow the characterization of new alleles and development of molecular markers associated with drought responses in peanut. These together constitute important tools for the peanut breeding program and also contribute to a better comprehension of gene modulation in response to water deficit and rehydration. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 59 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 59 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 19 | 32% |
| Student > Ph. D. Student | 12 | 20% |
| Student > Master | 3 | 5% |
| Student > Doctoral Student | 2 | 3% |
| Student > Bachelor | 2 | 3% |
| Other | 10 | 17% |
| Unknown | 11 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 34 | 58% |
| Biochemistry, Genetics and Molecular Biology | 7 | 12% |
| Unspecified | 2 | 3% |
| Social Sciences | 1 | 2% |
| Unknown | 15 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 January 2016.
All research outputs
#15,353,264
of 22,837,982 outputs
Outputs from Plant Molecular Biology Reporter
#262
of 353 outputs
Outputs of similar age
#157,521
of 264,643 outputs
Outputs of similar age from Plant Molecular Biology Reporter
#3
of 7 outputs
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