| Title |
Adipose tissue transcriptomics and epigenomics in low birthweight men and controls: role of high-fat overfeeding
|
|---|---|
| Published in |
Diabetologia, January 2016
|
| DOI | 10.1007/s00125-015-3852-9 |
| Pubmed ID | |
| Authors |
Linn Gillberg, Alexander Perfilyev, Charlotte Brøns, Martin Thomasen, Louise G. Grunnet, Petr Volkov, Fredrik Rosqvist, David Iggman, Ingrid Dahlman, Ulf Risérus, Tina Rönn, Emma Nilsson, Allan Vaag, Charlotte Ling |
| Abstract |
Individuals who had a low birthweight (LBW) are at an increased risk of insulin resistance and type 2 diabetes when exposed to high-fat overfeeding (HFO). We studied genome-wide mRNA expression and DNA methylation in subcutaneous adipose tissue (SAT) after 5 days of HFO and after a control diet in 40 young men, of whom 16 had LBW. mRNA expression was analysed using Affymetrix Human Gene 1.0 ST arrays and DNA methylation using Illumina 450K BeadChip arrays. We found differential DNA methylation at 53 sites in SAT from LBW vs normal birthweight (NBW) men (false discovery rate <5%), including sites in the FADS2 and CPLX1 genes previously associated with type 2 diabetes. When we used reference-free cell mixture adjustments to potentially adjust for cell composition, 4,323 sites had differential methylation in LBW vs NBW men. However, no differences in SAT gene expression levels were identified between LBW and NBW men. In the combined group of all 40 participants, 3,276 genes (16.5%) were differentially expressed in SAT after HFO (false discovery rate <5%) and there was no difference between LBW men and controls. The most strongly upregulated genes were ELOVL6, FADS2 and NNAT; in contrast, INSR, IRS2 and the SLC27A2 fatty acid transporter showed decreased expression after HFO. Interestingly, SLC27A2 expression correlated negatively with diabetes- and obesity-related traits in a replication cohort of 142 individuals. DNA methylation at 652 CpG sites (including in CDK5, IGFBP5 and SLC2A4) was altered in SAT after overfeeding in this and in another cohort. Young men who had a LBW exhibit epigenetic alterations in their adipose tissue that potentially influence insulin resistance and risk of type 2 diabetes. Short-term overfeeding influences gene transcription and, to some extent, DNA methylation in adipose tissue; there was no major difference in this response between LBW and control participants. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 9% |
| Venezuela, Bolivarian Republic of | 1 | 9% |
| Sweden | 1 | 9% |
| France | 1 | 9% |
| Unknown | 7 | 64% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 8 | 73% |
| Scientists | 2 | 18% |
| Science communicators (journalists, bloggers, editors) | 1 | 9% |
Mendeley readers
The data shown below were compiled from readership statistics for 102 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Mexico | 1 | <1% |
| United States | 1 | <1% |
| Portugal | 1 | <1% |
| Unknown | 99 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 18 | 18% |
| Student > Master | 15 | 15% |
| Researcher | 10 | 10% |
| Student > Bachelor | 10 | 10% |
| Student > Postgraduate | 6 | 6% |
| Other | 19 | 19% |
| Unknown | 24 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 22 | 22% |
| Medicine and Dentistry | 22 | 22% |
| Agricultural and Biological Sciences | 11 | 11% |
| Nursing and Health Professions | 6 | 6% |
| Neuroscience | 4 | 4% |
| Other | 12 | 12% |
| Unknown | 25 | 25% |
Attention Score in Context
This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 April 2016.
All research outputs
#5,657,994
of 22,837,982 outputs
Outputs from Diabetologia
#2,395
of 5,035 outputs
Outputs of similar age
#90,866
of 394,936 outputs
Outputs of similar age from Diabetologia
#25
of 67 outputs
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