| Title |
Establishment of a Chinook salmon cell line with an inducible gene expression system
|
|---|---|
| Published in |
In Vitro Cellular & Developmental Biology - Animal, November 2011
|
| DOI | 10.1007/s11626-011-9466-8 |
| Pubmed ID | |
| Authors |
Bertrand Collet, Katherine Lester |
| Abstract |
We have isolated a stable recombinant cell line CHSE-TOF5 derived from the Chinook salmon (Oncorhynchus tshawytscha) embryo cells for use as an inducible expression system. The cells were transfected with the pTet-Off plasmid from the Tet On/Off Clontech system, carrying a G418 resistance gene. Several G418-resistant clones were subcultured and characterised by quantitative PCR (qPCR) and by transient transfection. The level of expression of transcriptional activator was measured by qPCR in a number of isolated clones, and transient transfection with a pTRE2-hyg-LUC plasmid was used to evaluate the inducibility of these clones. A clone was selected for its relative fast cell growth and good level of inducibility. This genetically engineered cell line is a valuable tool for the fish research community especially in research areas investigating the biological function of proteins from fish or fish pathogens. |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 5 | 24% |
| Student > Ph. D. Student | 5 | 24% |
| Student > Bachelor | 3 | 14% |
| Student > Master | 3 | 14% |
| Other | 1 | 5% |
| Other | 2 | 10% |
| Unknown | 2 | 10% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 7 | 33% |
| Agricultural and Biological Sciences | 6 | 29% |
| Immunology and Microbiology | 2 | 10% |
| Unspecified | 1 | 5% |
| Veterinary Science and Veterinary Medicine | 1 | 5% |
| Other | 1 | 5% |
| Unknown | 3 | 14% |