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The murine neutrophil NLRP3 inflammasome is activated by soluble but not particulate or crystalline agonists

Overview of attention for article published in European Journal of Immunology, February 2016
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  • In the top 25% of all research outputs scored by Altmetric
  • High Attention Score compared to outputs of the same age (85th percentile)
  • Good Attention Score compared to outputs of the same age and source (68th percentile)

Mentioned by

news
1 news outlet
wikipedia
1 Wikipedia page

Citations

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23 Dimensions

Readers on

mendeley
43 Mendeley
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Title
The murine neutrophil NLRP3 inflammasome is activated by soluble but not particulate or crystalline agonists
Published in
European Journal of Immunology, February 2016
DOI 10.1002/eji.201545943
Pubmed ID
Authors

Kaiwen W. Chen, Jelena S. Bezbradica, Christina J. Groß, Adam A. Wall, Matthew J. Sweet, Jennifer L. Stow, Kate Schroder

Abstract

Neutrophils express pattern recognition receptors (PRRs) and regulate immune responses via PRR-dependent cytokine production. An emerging theme is that neutrophil PRRs often exhibit cell type-specific adaptations in their signalling pathways. This prompted us to examine inflammasome signalling by the PRR NLRP3 in murine neutrophils, in comparison to well-established NLRP3 signalling pathways in macrophages. Here, we demonstrate that while murine neutrophils can indeed signal via the NLRP3 inflammasome, neutrophil NLRP3 selectively responds to soluble agonists but not to the particulate/crystalline agonists that trigger NLRP3 activation in macrophages via phagolysosomal rupture. In keeping with this, alum did not trigger IL-1β production from human PMN, and the lysosomotropic peptide Leu-Leu-OMe stimulated only weak NLRP3-dependent IL-1β production from murine neutrophils, suggesting that lysosomal rupture is not a strong stimulus for NLRP3 activation in neutrophils. We validated our in vitro findings for poor neutrophil NLRP3 responses to particles in vivo, where we demonstrated that neutrophils do not significantly contribute to alum-induced IL-1β production in mice. In all, our studies highlight that myeloid cell identity and the nature of the danger signal can strongly influence signalling by a single PRR, thus shaping the nature of the resultant immune response.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 43 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Japan 1 2%
Brazil 1 2%
Unknown 41 95%

Demographic breakdown

Readers by professional status Count As %
Researcher 8 19%
Student > Master 7 16%
Student > Bachelor 6 14%
Student > Doctoral Student 5 12%
Student > Ph. D. Student 2 5%
Other 5 12%
Unknown 10 23%
Readers by discipline Count As %
Immunology and Microbiology 11 26%
Agricultural and Biological Sciences 7 16%
Biochemistry, Genetics and Molecular Biology 6 14%
Medicine and Dentistry 4 9%
Pharmacology, Toxicology and Pharmaceutical Science 2 5%
Other 4 9%
Unknown 9 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 July 2019.
All research outputs
#2,944,772
of 22,840,638 outputs
Outputs from European Journal of Immunology
#377
of 6,591 outputs
Outputs of similar age
#54,669
of 397,367 outputs
Outputs of similar age from European Journal of Immunology
#18
of 67 outputs
Altmetric has tracked 22,840,638 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 6,591 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.0. This one has done particularly well, scoring higher than 93% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 397,367 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 67 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 68% of its contemporaries.