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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Microwell perfusion array for high-throughput, long-term imaging of clonal growth
|
|---|---|
| Published in |
Biomicrofluidics, December 2011
|
| DOI | 10.1063/1.3669371 |
| Pubmed ID | |
| Authors |
Huaying Chen, Jingjing Li, Han Zhang, Musen Li, Gary Rosengarten, Robert E. Nordon |
| Abstract |
Continuous cell tracking by time-lapse microscopy has led to detailed study of cell differentiation pathways using single cell fate maps. There are a multitude of cell fate outcomes, so hundreds of clonal division histories are required to measure these stochastic branching processes. This study examines the principle of condensing cell imaging information into a relatively small region to maximize live cell imaging throughput. High throughput clonal analysis of non-adherent cells by continuous live cell tracking was possible using a microwell perfusion array with an internal volume of 16 μl and 600 microwells at the base. This study includes examination of biocompatibility of buffer systems, connecting tubing, cell culture substrates, and media degradation. An intermittent perfusion protocol was selected for long-term time-lapse imaging of KG1a cells in the microwell array; 1500 clones were simultaneously cultured and scanned every 3 min at 100 × magnifications for 6 days. The advantages of perfusion microwell culture are continuous long-term cell tracking, higher cell imaging throughput, and greater control over cell microenvironment. Microwell devices facilitate high throughput analysis of cell lineage development and measurement of the probability distribution for cell life events such as mitosis. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Peru | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Science communicators (journalists, bloggers, editors) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 3 | 11% |
| United States | 1 | 4% |
| Finland | 1 | 4% |
| Unknown | 23 | 82% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 8 | 29% |
| Student > Master | 7 | 25% |
| Student > Ph. D. Student | 6 | 21% |
| Student > Doctoral Student | 3 | 11% |
| Professor > Associate Professor | 2 | 7% |
| Other | 0 | 0% |
| Unknown | 2 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Engineering | 12 | 43% |
| Agricultural and Biological Sciences | 7 | 25% |
| Biochemistry, Genetics and Molecular Biology | 3 | 11% |
| Medicine and Dentistry | 2 | 7% |
| Computer Science | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 3 | 11% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 16 January 2012.
All research outputs
#20,657,128
of 25,377,790 outputs
Outputs from Biomicrofluidics
#604
of 833 outputs
Outputs of similar age
#204,627
of 249,054 outputs
Outputs of similar age from Biomicrofluidics
#8
of 22 outputs
Altmetric has tracked 25,377,790 research outputs across all sources so far. This one is in the 10th percentile – i.e., 10% of other outputs scored the same or lower than it.
So far Altmetric has tracked 833 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.5. This one is in the 15th percentile – i.e., 15% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 249,054 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 8th percentile – i.e., 8% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 22 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.