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X Demographics
Mendeley readers
Attention Score in Context
| Title |
A novel method of microsatellite genotyping-by-sequencing using individual combinatorial barcoding
|
|---|---|
| Published in |
Royal Society Open Science, January 2016
|
| DOI | 10.1098/rsos.150565 |
| Pubmed ID | |
| Authors |
Salla Vartia, José L. Villanueva-Cañas, John Finarelli, Edward D. Farrell, Patrick C. Collins, Graham M. Hughes, Jeanette E. L. Carlsson, David T. Gauthier, Philip McGinnity, Thomas F. Cross, Richard D. FitzGerald, Luca Mirimin, Fiona Crispie, Paul D. Cotter, Jens Carlsson |
| Abstract |
This study examines the potential of next-generation sequencing based 'genotyping-by-sequencing' (GBS) of microsatellite loci for rapid and cost-effective genotyping in large-scale population genetic studies. The recovery of individual genotypes from large sequence pools was achieved by PCR-incorporated combinatorial barcoding using universal primers. Three experimental conditions were employed to explore the possibility of using this approach with existing and novel multiplex marker panels and weighted amplicon mixture. The GBS approach was validated against microsatellite data generated by capillary electrophoresis. GBS allows access to the underlying nucleotide sequences that can reveal homoplasy, even in large datasets and facilitates cross laboratory transfer. GBS of microsatellites, using individual combinatorial barcoding, is potentially faster and cheaper than current microsatellite approaches and offers better and more data. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Ireland | 2 | 33% |
| Unknown | 4 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 4 | 67% |
| Scientists | 2 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 197 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Italy | 2 | 1% |
| Spain | 1 | <1% |
| France | 1 | <1% |
| Canada | 1 | <1% |
| Unknown | 192 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 47 | 24% |
| Student > Ph. D. Student | 35 | 18% |
| Student > Master | 24 | 12% |
| Student > Bachelor | 18 | 9% |
| Student > Postgraduate | 9 | 5% |
| Other | 34 | 17% |
| Unknown | 30 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 94 | 48% |
| Biochemistry, Genetics and Molecular Biology | 42 | 21% |
| Environmental Science | 14 | 7% |
| Social Sciences | 4 | 2% |
| Engineering | 4 | 2% |
| Other | 7 | 4% |
| Unknown | 32 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 10. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 March 2023.
All research outputs
#3,187,344
of 23,495,502 outputs
Outputs from Royal Society Open Science
#1,784
of 4,207 outputs
Outputs of similar age
#56,153
of 396,881 outputs
Outputs of similar age from Royal Society Open Science
#41
of 82 outputs
Altmetric has tracked 23,495,502 research outputs across all sources so far. Compared to these this one has done well and is in the 86th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,207 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 50.1. This one has gotten more attention than average, scoring higher than 57% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 396,881 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 85% of its contemporaries.
We're also able to compare this research output to 82 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.