| Title |
Proteomics analysis of global regulatory cascades involved in clavulanic acid production and morphological development in Streptomyces clavuligerus
|
|---|---|
| Published in |
Journal of Industrial Microbiology & Biotechnology, April 2016
|
| DOI | 10.1007/s10295-016-1733-y |
| Pubmed ID | |
| Authors |
Nicole L Ferguson, Lourdes Peña-Castillo, Marcus A Moore, Dawn R D Bignell, Kapil Tahlan |
| Abstract |
The genus Streptomyces comprises bacteria that undergo a complex developmental life cycle and produce many metabolites of importance to industry and medicine. Streptomyces clavuligerus produces the β-lactamase inhibitor clavulanic acid, which is used in combination with β-lactam antibiotics to treat certain β-lactam resistant bacterial infections. Many aspects of how clavulanic acid production is globally regulated in S. clavuligerus still remains unknown. We conducted comparative proteomics analysis using the wild type strain of S. clavuligerus and two mutants (ΔbldA and ΔbldG), which are defective in global regulators and vary in their ability to produce clavulanic acid. Approximately 33.5 % of the predicted S. clavuligerus proteome was detected and 192 known or putative regulatory proteins showed statistically differential expression levels in pairwise comparisons. Interestingly, the expression of many proteins whose corresponding genes contain TTA codons (predicted to require the bldA tRNA for translation) was unaffected in the bldA mutant. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Canada | 1 | 50% |
| United Kingdom | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 47 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 47 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 13 | 28% |
| Student > Master | 8 | 17% |
| Researcher | 6 | 13% |
| Student > Bachelor | 5 | 11% |
| Student > Doctoral Student | 3 | 6% |
| Other | 6 | 13% |
| Unknown | 6 | 13% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 15 | 32% |
| Biochemistry, Genetics and Molecular Biology | 14 | 30% |
| Engineering | 3 | 6% |
| Chemical Engineering | 2 | 4% |
| Chemistry | 2 | 4% |
| Other | 5 | 11% |
| Unknown | 6 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 December 2018.
All research outputs
#16,720,137
of 25,371,288 outputs
Outputs from Journal of Industrial Microbiology & Biotechnology
#1,270
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Outputs of similar age
#183,153
of 314,718 outputs
Outputs of similar age from Journal of Industrial Microbiology & Biotechnology
#15
of 32 outputs
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