| Title |
A Comparison of DESI-MS and LC-MS for the Lipidomic Profiling of Human Cancer Tissue
|
|---|---|
| Published in |
Journal of the American Society for Mass Spectrometry, October 2015
|
| DOI | 10.1007/s13361-015-1278-8 |
| Pubmed ID | |
| Authors |
Nima Abbassi-Ghadi, Emrys A. Jones, Maria Gomez-Romero, Ottmar Golf, Sacheen Kumar, Juzheng Huang, Hiromi Kudo, Rob D. Goldin, George B. Hanna, Zoltan Takats |
| Abstract |
In this study, we make a direct comparison between desorption electrospray ionization-mass spectrometry (DESI-MS) and ultraperformance liquid chromatography-electrospray ionization-mass spectrometry (UPLC-ESI-MS) platforms for the profiling of glycerophospholipid (GPL) species in esophageal cancer tissue. In particular, we studied the similarities and differences in the range of GPLs detected and the congruency of their relative abundances as detected by each analytical platform. The main differences between mass spectra of the two modalities were found to be associated with the variance in adduct formation of common GPLs, rather than the presence of different GPL species. Phosphatidylcholines as formate adducts in UPLC-ESI-MS accounted for the majority of differences in negative ion mode and alkali metal adducts of phosphatidylcholines in DESI-MS for positive ion mode. Comparison of the relative abundance of GPLs, normalized to a common peak, revealed a correlation coefficient of 0.70 (P < 0.001). The GPL profile detected by DESI-MS is congruent to UPLC-ESI-MS, which reaffirms the role of DESI-MS for lipidomic profiling and a potential premise for quantification. Graphical Abstract ᇵ. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| India | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 76 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Unknown | 75 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 18 | 24% |
| Researcher | 13 | 17% |
| Student > Master | 10 | 13% |
| Other | 8 | 11% |
| Student > Doctoral Student | 6 | 8% |
| Other | 9 | 12% |
| Unknown | 12 | 16% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 22 | 29% |
| Biochemistry, Genetics and Molecular Biology | 11 | 14% |
| Agricultural and Biological Sciences | 8 | 11% |
| Medicine and Dentistry | 8 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 5% |
| Other | 10 | 13% |
| Unknown | 13 | 17% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 January 2016.
All research outputs
#20,656,820
of 25,374,647 outputs
Outputs from Journal of the American Society for Mass Spectrometry
#3,083
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Outputs of similar age
#213,239
of 291,148 outputs
Outputs of similar age from Journal of the American Society for Mass Spectrometry
#19
of 26 outputs
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