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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Botulinum neurotoxin type-A enters a non-recycling pool of synaptic vesicles
|
|---|---|
| Published in |
Scientific Reports, January 2016
|
| DOI | 10.1038/srep19654 |
| Pubmed ID | |
| Authors |
Callista B. Harper, Andreas Papadopulos, Sally Martin, Daniel R. Matthews, Garry P. Morgan, Tam H. Nguyen, Tong Wang, Deepak Nair, Daniel Choquet, Frederic A. Meunier |
| Abstract |
Neuronal communication relies on synaptic vesicles undergoing regulated exocytosis and recycling for multiple rounds of fusion. Whether all synaptic vesicles have identical protein content has been challenged, suggesting that their recycling ability may differ greatly. Botulinum neurotoxin type-A (BoNT/A) is a highly potent neurotoxin that is internalized in synaptic vesicles at motor nerve terminals and induces flaccid paralysis. Recently, BoNT/A was also shown to undergo retrograde transport, suggesting it might enter a specific pool of synaptic vesicles with a retrograde trafficking fate. Using high-resolution microscopy techniques including electron microscopy and single molecule imaging, we found that the BoNT/A binding domain is internalized within a subset of vesicles that only partially co-localize with cholera toxin B-subunit and have markedly reduced VAMP2 immunoreactivity. Synaptic vesicles loaded with pHrodo-BoNT/A-Hc exhibited a significantly reduced ability to fuse with the plasma membrane in mouse hippocampal nerve terminals when compared with pHrodo-dextran-containing synaptic vesicles and pHrodo-labeled anti-GFP nanobodies bound to VAMP2-pHluorin or vGlut-pHluorin. Similar results were also obtained at the amphibian neuromuscular junction. These results reveal that BoNT/A is internalized in a subpopulation of synaptic vesicles that are not destined to recycle, highlighting the existence of significant molecular and functional heterogeneity between synaptic vesicles. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Germany | 1 | 2% |
| France | 1 | 2% |
| Australia | 1 | 2% |
| Switzerland | 1 | 2% |
| Unknown | 57 | 93% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 20% |
| Student > Ph. D. Student | 11 | 18% |
| Student > Master | 10 | 16% |
| Student > Doctoral Student | 6 | 10% |
| Other | 4 | 7% |
| Other | 9 | 15% |
| Unknown | 9 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 16 | 26% |
| Agricultural and Biological Sciences | 12 | 20% |
| Biochemistry, Genetics and Molecular Biology | 6 | 10% |
| Medicine and Dentistry | 6 | 10% |
| Veterinary Science and Veterinary Medicine | 2 | 3% |
| Other | 9 | 15% |
| Unknown | 10 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 January 2016.
All research outputs
#14,245,321
of 22,842,950 outputs
Outputs from Scientific Reports
#67,531
of 123,340 outputs
Outputs of similar age
#207,895
of 396,496 outputs
Outputs of similar age from Scientific Reports
#1,804
of 3,212 outputs
Altmetric has tracked 22,842,950 research outputs across all sources so far. This one is in the 35th percentile – i.e., 35% of other outputs scored the same or lower than it.
So far Altmetric has tracked 123,340 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 18.2. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 396,496 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 44th percentile – i.e., 44% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 3,212 others from the same source and published within six weeks on either side of this one. This one is in the 40th percentile – i.e., 40% of its contemporaries scored the same or lower than it.