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Measurement of haem and total iron in fish, shrimp and prawn using ICP-MS: Implications for dietary iron intake calculations

Overview of attention for article published in Food Chemistry, January 2016
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Title
Measurement of haem and total iron in fish, shrimp and prawn using ICP-MS: Implications for dietary iron intake calculations
Published in
Food Chemistry, January 2016
DOI 10.1016/j.foodchem.2016.01.080
Pubmed ID
Authors

Matthew S. Wheal, Emma DeCourcy-Ireland, Jessica R. Bogard, Shakuntala H. Thilsted, James C.R. Stangoulis

Abstract

Twenty-five species of fish, shrimp and prawn from local markets in Bangladesh were analysed for concentrations of total Fe, haem Fe and non-haem Fe by ICP-MS. Total Fe and non-haem Fe concentrations were measured in nitric acid-digested samples and haem Fe was extracted using acidified 80% acetone for 60min. Total Fe concentrations ranged from 0.55-14.43mg/100g FW, and haem Fe% ranged from 18%-93% of total Fe. Repeat extractions with 80% acetone recovered additional haem Fe, suggesting that previous measurement by this technique may have underestimated haem Fe content. Calculation of Fe balance (summing Fe in acetone extracts and Fe in the residue after haem Fe extraction) was not significantly different from total Fe, indicating the two processes recovered the different forms of Fe with similar effectiveness.

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Geographical breakdown

Country Count As %
Unknown 85 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 16 19%
Student > Ph. D. Student 11 13%
Student > Master 10 12%
Researcher 9 11%
Student > Doctoral Student 4 5%
Other 14 16%
Unknown 21 25%
Readers by discipline Count As %
Agricultural and Biological Sciences 18 21%
Environmental Science 11 13%
Chemistry 9 11%
Engineering 5 6%
Biochemistry, Genetics and Molecular Biology 4 5%
Other 12 14%
Unknown 26 31%