| Title |
Effect of biosurfactants on Pseudomonas aeruginosa and Staphylococcus aureus biofilms in a BioFlux channel
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, January 2016
|
| DOI | 10.1007/s00253-016-7310-5 |
| Pubmed ID | |
| Authors |
M. A. Diaz De Rienzo, P. S. Stevenson, R. Marchant, I. M. Banat |
| Abstract |
Recent studies have indicated that biosurfactants play a role both in maintaining channels between multicellular structures in biofilms and in dispersal of cells from biofilms. A combination of caprylic acid (0.01 % v/v) together with rhamnolipids (0.04 % v/v) was applied to biofilms of Pseudomonas aeruginosa ATCC 15442, Staphylococcus aureus ATCC 9144 and a mixed culture under BioFlux flowthrough conditions and caused disruption of the biofilms. The biofilms were also treated with a combination of rhamnolipids (0.04 % v/v) and sophorolipids (0.01 %). Control treatments with PBS 1× had no apparent effect on biofilm disruption. The Gram-positive bacterium (S. aureus ATCC 9144) was more sensitive than P. aeruginosa ATCC 15442 in terms of disruption and viability as shown by Live/Dead staining. Disruption of biofilms of P. aeruginosa ATCC 15442 was minimal. Oxygen consumption by biofilms, after different treatments with biosurfactants, confirms that sophorolipid on its own is unable to kill/inhibit cells of P. aeruginosa ATCC 15442, and even when used in combination with rhamnolipids, under static conditions, no decrease in the cell viability was observed. Cells in biofilms exposed to mono-rhamnolipids (0.04 % v/v) showed behaviour typical of exposure to bacteriostatic compounds, but when exposed to di-rhamnolipids (0.04 % v/v), they displayed a pattern characteristic of bactericidal compounds. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 131 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Brazil | 1 | <1% |
| Unknown | 130 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 24 | 18% |
| Researcher | 19 | 15% |
| Student > Bachelor | 12 | 9% |
| Student > Master | 11 | 8% |
| Student > Doctoral Student | 8 | 6% |
| Other | 22 | 17% |
| Unknown | 35 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 30 | 23% |
| Biochemistry, Genetics and Molecular Biology | 22 | 17% |
| Immunology and Microbiology | 13 | 10% |
| Medicine and Dentistry | 6 | 5% |
| Environmental Science | 6 | 5% |
| Other | 19 | 15% |
| Unknown | 35 | 27% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 31 January 2016.
All research outputs
#15,827,358
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#5,634
of 8,034 outputs
Outputs of similar age
#229,193
of 404,533 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#70
of 127 outputs
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So far Altmetric has tracked 8,034 research outputs from this source. They receive a mean Attention Score of 4.3. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
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We're also able to compare this research output to 127 others from the same source and published within six weeks on either side of this one. This one is in the 42nd percentile – i.e., 42% of its contemporaries scored the same or lower than it.