| Title |
Role of malate transporter in lipid accumulation of oleaginous fungus Mucor circinelloides
|
|---|---|
| Published in |
Applied Microbiology and Biotechnology, October 2015
|
| DOI | 10.1007/s00253-015-7079-y |
| Pubmed ID | |
| Authors |
Lina Zhao, José T. Cánovas-Márquez, Xin Tang, Haiqin Chen, Yong Q. Chen, Wei Chen, Victoriano Garre, Yuanda Song, Colin Ratledge |
| Abstract |
Fatty acid biosynthesis in oleaginous fungi requires the supply of reducing power, NADPH, and the precursor of fatty acids, acetyl-CoA, which is generated in the cytosol being produced by ATP: citrate lyase which requires citrate to be, transported from the mitochondrion by the citrate/malate/pyruvate transporter. This transporter, which is within the mitochondrial membrane, transports cytosolic malate into the mitochondrion in exchange for mitochondrial citrate moving into the cytosol (Fig. 1). The role of malate transporter in lipid accumulation in oleaginous fungi is not fully understood, however. Therefore, the expression level of the mt gene, coding for a malate transporter, was manipulated in the oleaginous fungus Mucor circinelloides to analyze its effect on lipid accumulation. The results showed that mt overexpression increased the lipid content for about 70 % (from 13 to 22 % dry cell weight, CDW), whereas the lipid content in mt knockout mutant decreased about 27 % (from 13 to 9.5 % CDW) compared with the control strain. Furthermore, the extracellular malate concentration was decreased in the mt overexpressing strain and increased in the mt knockout strain compared with the wild-type strain. This work suggests that the malate transporter plays an important role in regulating lipid accumulation in oleaginous fungus M. circinelloides. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | 3% |
| Spain | 1 | 3% |
| Sweden | 1 | 3% |
| China | 1 | 3% |
| Unknown | 35 | 90% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 21% |
| Student > Master | 6 | 15% |
| Researcher | 4 | 10% |
| Student > Bachelor | 3 | 8% |
| Other | 3 | 8% |
| Other | 7 | 18% |
| Unknown | 8 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 14 | 36% |
| Agricultural and Biological Sciences | 9 | 23% |
| Immunology and Microbiology | 2 | 5% |
| Chemistry | 2 | 5% |
| Chemical Engineering | 1 | 3% |
| Other | 1 | 3% |
| Unknown | 10 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 February 2016.
All research outputs
#14,682,212
of 24,119,703 outputs
Outputs from Applied Microbiology and Biotechnology
#5,377
of 8,034 outputs
Outputs of similar age
#144,535
of 289,449 outputs
Outputs of similar age from Applied Microbiology and Biotechnology
#51
of 139 outputs
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