You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
X Demographics
Mendeley readers
Attention Score in Context
| Title |
Therapeutic genome editing by combined viral and non-viral delivery of CRISPR system components in vivo
|
|---|---|
| Published in |
Nature Biotechnology, February 2016
|
| DOI | 10.1038/nbt.3471 |
| Pubmed ID | |
| Authors |
Hao Yin, Chun-Qing Song, Joseph R Dorkin, Lihua J Zhu, Yingxiang Li, Qiongqiong Wu, Angela Park, Junghoon Yang, Sneha Suresh, Aizhan Bizhanova, Ankit Gupta, Mehmet F Bolukbasi, Stephen Walsh, Roman L Bogorad, Guangping Gao, Zhiping Weng, Yizhou Dong, Victor Koteliansky, Scot A Wolfe, Robert Langer, Wen Xue, Daniel G Anderson |
| Abstract |
The combination of Cas9, guide RNA and repair template DNA can induce precise gene editing and the correction of genetic diseases in adult mammals. However, clinical implementation of this technology requires safe and effective delivery of all of these components into the nuclei of the target tissue. Here, we combine lipid nanoparticle-mediated delivery of Cas9 mRNA with adeno-associated viruses encoding a sgRNA and a repair template to induce repair of a disease gene in adult animals. We applied our delivery strategy to a mouse model of human hereditary tyrosinemia and show that the treatment generated fumarylacetoacetate hydrolase (Fah)-positive hepatocytes by correcting the causative Fah-splicing mutation. Treatment rescued disease symptoms such as weight loss and liver damage. The efficiency of correction was >6% of hepatocytes after a single application, suggesting potential utility of Cas9-based therapeutic genome editing for a range of diseases. |
X Demographics
The data shown below were collected from the profiles of 66 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 17 | 26% |
| United Kingdom | 4 | 6% |
| France | 4 | 6% |
| Japan | 3 | 5% |
| Belgium | 2 | 3% |
| Germany | 2 | 3% |
| Austria | 1 | 2% |
| Australia | 1 | 2% |
| Denmark | 1 | 2% |
| Other | 3 | 5% |
| Unknown | 28 | 42% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 38 | 58% |
| Scientists | 26 | 39% |
| Science communicators (journalists, bloggers, editors) | 2 | 3% |
Mendeley readers
The data shown below were compiled from readership statistics for 1,099 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 8 | <1% |
| Germany | 3 | <1% |
| Finland | 2 | <1% |
| Turkey | 2 | <1% |
| Spain | 2 | <1% |
| Netherlands | 1 | <1% |
| China | 1 | <1% |
| Canada | 1 | <1% |
| Belgium | 1 | <1% |
| Other | 1 | <1% |
| Unknown | 1077 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 234 | 21% |
| Researcher | 174 | 16% |
| Student > Bachelor | 154 | 14% |
| Student > Master | 122 | 11% |
| Student > Doctoral Student | 55 | 5% |
| Other | 145 | 13% |
| Unknown | 215 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 328 | 30% |
| Agricultural and Biological Sciences | 219 | 20% |
| Engineering | 55 | 5% |
| Medicine and Dentistry | 50 | 5% |
| Chemistry | 42 | 4% |
| Other | 152 | 14% |
| Unknown | 253 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 190. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2024.
All research outputs
#215,971
of 25,954,278 outputs
Outputs from Nature Biotechnology
#442
of 8,655 outputs
Outputs of similar age
#3,716
of 408,975 outputs
Outputs of similar age from Nature Biotechnology
#13
of 118 outputs
Altmetric has tracked 25,954,278 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 99th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 8,655 research outputs from this source. They typically receive a lot more attention than average, with a mean Attention Score of 44.6. This one has done particularly well, scoring higher than 94% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 408,975 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 99% of its contemporaries.
We're also able to compare this research output to 118 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 88% of its contemporaries.