| Title |
Huh-7 cell line as an alternative cultural model for the production of human like erythropoietin (EPO)
|
|---|---|
| Published in |
Journal of Translational Medicine, November 2011
|
| DOI | 10.1186/1479-5876-9-186 |
| Pubmed ID | |
| Authors |
Humera Kausar, Sana Gull, Bushra Ijaz, Waqar Ahmad, Muhammad Tahir Sarwar, Zafar Iqbal, Zafar Nawaz, Sheikh Riazuddin, Sajida Hassan |
| Abstract |
Erythropoietin (EPO) is a glycoprotein hormone which is required to regulate the production of red blood cells. Deficiency of EPO is known to cause anemia in chronically infected renal patients and they require regular blood transfusion. Availability of recombinant EPO has eliminated the need for blood transfusion and now it is extensively used for the treatment of anemia. Glycosylation of erythropoietin is essential for its secretion, stability, protein conformation and biological activity. However, maintenance of human like glycosylation pattern during manufacturing of EPO is a major challenge in biotechnology. Currently, Chinese hamster ovary (CHO) cell line is used for the commercial production of erythropoietin but this cell line does not maintain glycosylation resembling human system. With the trend to eliminate non-human constituent from biopharmaceutical products, as a preliminary approach, we have investigated the potential of human hepatoma cell line (Huh-7) to produce recombinant EPO. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 49 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 49 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 10 | 20% |
| Researcher | 6 | 12% |
| Student > Doctoral Student | 4 | 8% |
| Student > Master | 4 | 8% |
| Other | 2 | 4% |
| Other | 6 | 12% |
| Unknown | 17 | 35% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 20% |
| Biochemistry, Genetics and Molecular Biology | 7 | 14% |
| Medicine and Dentistry | 4 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 3 | 6% |
| Chemical Engineering | 2 | 4% |
| Other | 3 | 6% |
| Unknown | 20 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 December 2011.
All research outputs
#15,240,835
of 22,660,862 outputs
Outputs from Journal of Translational Medicine
#2,211
of 3,951 outputs
Outputs of similar age
#96,413
of 141,732 outputs
Outputs of similar age from Journal of Translational Medicine
#33
of 52 outputs
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