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Nonribosomal Peptide and Polyketide Biosynthesis

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Cover of 'Nonribosomal Peptide and Polyketide Biosynthesis'

Table of Contents

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    Book Overview
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    Chapter 1 Structural Biology of Nonribosomal Peptide Synthetases.
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    Chapter 2 The Assembly Line Enzymology of Polyketide Biosynthesis.
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    Chapter 3 Measurement of Nonribosomal Peptide Synthetase Adenylation Domain Activity Using a Continuous Hydroxylamine Release Assay
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    Chapter 4 Affinity Purification Method for the Identification of Nonribosomal Peptide Biosynthetic Enzymes Using a Synthetic Probe for Adenylation Domains
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    Chapter 5 Colorimetric Detection of the Adenylation Activity in Nonribosomal Peptide Synthetases
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    Chapter 6 Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis
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    Chapter 7 Reconstitution of Fungal Nonribosomal Peptide Synthetases in Yeast and In Vitro.
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    Chapter 8 The Continuing Development of E. coli as a Heterologous Host for Complex Natural Product Biosynthesis
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    Chapter 9 Screening for Expressed Nonribosomal Peptide Synthetases and Polyketide Synthases Using LC-MS/MS-Based Proteomics.
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    Chapter 10 Enhancing Nonribosomal Peptide Biosynthesis in Filamentous Fungi.
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    Chapter 11 In Situ Analysis of Bacterial Lipopeptide Antibiotics by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging.
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    Chapter 12 Secondary Metabolic Pathway-Targeted Metabolomics.
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    Chapter 13 Annotating and Interpreting Linear and Cyclic Peptide Tandem Mass Spectra.
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    Chapter 14 Bioinformatics Tools for the Discovery of New Nonribosomal Peptides
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    Chapter 15 The Use of ClusterMine360 for the Analysis of Polyketide and Nonribosomal Peptide Biosynthetic Pathways.
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    Chapter 16 Alignment-Free Methods for the Detection and Specificity Prediction of Adenylation Domains.
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    Chapter 17 Characterization of Nonribosomal Peptide Synthetases with NRPSsp.
Attention for Chapter 17: Characterization of Nonribosomal Peptide Synthetases with NRPSsp.
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Chapter title
Characterization of Nonribosomal Peptide Synthetases with NRPSsp.
Chapter number 17
Book title
Nonribosomal Peptide and Polyketide Biosynthesis
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3375-4_17
Pubmed ID
Book ISBNs
978-1-4939-3373-0, 978-1-4939-3375-4
Authors

Prieto, Carlos, Carlos Prieto

Abstract

Bioinformatic sequence analysis allows the functional characterization of newly sequenced proteins. Nonribosomal peptide synthetases (NRPSs) are multi-modular enzymes involved in the biosynthesis of natural products. The current omics era has enabled the exponential growth of the sequenced NRPS, and it is important to characterize the final product of these synthetases. Here, how to achieve the prediction of substrates which bind to adenylation domains in NRPS with NRPSsp ( www.nrpssp.com ) bioinformatic tool is described.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 11%
Unknown 8 89%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 33%
Researcher 2 22%
Student > Bachelor 1 11%
Student > Master 1 11%
Student > Doctoral Student 1 11%
Other 0 0%
Unknown 1 11%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 2 22%
Agricultural and Biological Sciences 2 22%
Chemistry 2 22%
Medicine and Dentistry 1 11%
Immunology and Microbiology 1 11%
Other 0 0%
Unknown 1 11%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 03 February 2016.
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#18,437,241
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Outputs of similar age from Methods in molecular biology
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