Title |
Time-Series Transcriptomics Reveals That AGAMOUS-LIKE22 Affects Primary Metabolism and Developmental Processes in Drought-Stressed Arabidopsis
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Published in |
Plant Cell, February 2016
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DOI | 10.1105/tpc.15.00910 |
Pubmed ID | |
Authors |
Ulrike Bechtold, Christopher A. Penfold, Dafyd J. Jenkins, Roxane Legaie, Jonathan D. Moore, Tracy Lawson, Jack S.A. Matthews, Silvere R.M. Vialet-Chabrand, Laura Baxter, Sunitha Subramaniam, Richard Hickman, Hannah Florance, Christine Sambles, Deborah L. Salmon, Regina Feil, Laura Bowden, Claire Hill, Neil R. Baker, John E. Lunn, Bärbel Finkenstädt, Andrew Mead, Vicky Buchanan-Wollaston, Jim Beynon, David A. Rand, David L. Wild, Katherine J. Denby, Sascha Ott, Nicholas Smirnoff, Philip M. Mullineaux |
Abstract |
Water availability is the biggest single limitation on plant productivity worldwide. In Arabidopsis, changes in metabolism and gene expression drive increased drought tolerance and initiate diverse drought avoidance and escape responses. To address regulatory processes that link these responses together, we set out to identify novel genes that govern early responses to drought. To do this, a high-resolution time series transcriptomics dataset was produced, coupled with detailed physiological and metabolic analyses of plants subjected to a slow transition from well-watered to drought conditions. 1815 drought-responsive differentially expressed genes were identified. The major early changes in gene expression coincided with a drop in carbon assimilation, and only in the late stages with an increase in foliar abscisic acid content. In order to identify gene regulatory networks (GRNs) mediating the transition between the early and late stages of drought, we used Bayesian network modelling of differentially expressed transcription factor (TF) genes. This approach identified AGAMOUS-LIKE22 as key hub gene in a TF GRN. It has previously been shown that AGL22 is involved in the transition from vegetative state to flowering but here we show that AGL22 expression influences steady state photosynthetic rates and lifetime water use. This suggests that AGL22 uniquely regulates a transcriptional network during drought stress, linking changes in primary metabolism and the initiation of stress responses. |
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